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Anti-HEYL Antibody (aa26-54) LS-C166849

Catalog Size Price
LS-C166849-400 400 µl Unavailable

Related Products

23 HEYL Antibodies

Most Popular HEYL Antibodies

Anti-HEYL Antibody (aa34-47) LS-C24222
Rabbit Polyclonal (IgG) to Human HEYL
Human, Rodent
IHC, IHC - Frozen, Western blot, ELISA
Unconjugated
Anti-HEYL Antibody (N-Terminus) LS-C30789
Rabbit Polyclonal (IgG) to Human HEYL
Human, Monkey, Mouse, Rat, Guinea pig, Pig
Western blot
Unconjugated
Fetal Muscle Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HEYL Antibody (clone 1F6) LS-C133717
Mouse Monoclonal [clone 1F6] (IgG) to Human HEYL
Human
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunoperoxidase of monoclonal antibody to HEYL on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml]
Anti-HEYL Antibody (AP) LS-C508655
Rabbit Polyclonal (IgG) to Human HEYL
Human, Rodent
IHC, Western blot, ELISA
AP Conjugated
Anti-HEYL Antibody (APC) LS-C517267
Rabbit Polyclonal (IgG) to Human HEYL
Human, Rodent
IHC, Western blot, ELISA
APC Conjugated

100% Guaranteed
Rabbit Polyclonal to Human HEYL
Human
Immunofluorescence, Western blot
Unconjugated

Details

Human HEYL
Rabbit
Human (tested or 100% immunogen sequence identity)
Mouse, Cow (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)

Specificity and Use

LS-E4635 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C166849. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa26-54
This HEYL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-54 amino acids from the N-terminal region of human HEYL.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About HEYL

Q9NQ87 NM_014571 NP_055386.1

HEYL Antibody, HEY-like protein Antibody, HHRT3 Antibody, HRT-3 Antibody, HRT3 Antibody, HEY3 Antibody, BHLHb33 Antibody, HHeyL Antibody

Downstream effector of Notch signaling which may be required for cardiovascular development (By similarity). Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3' (By similarity). Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.

Immunofluorescence

Fluorescent confocal image of HeLa cell stained with HEYL Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HEYL primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HEYL immunoreactivity is localized to Mitochondria significantly.
Fluorescent confocal image of HeLa cell stained with HEYL Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HEYL primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HEYL immunoreactivity is localized to Mitochondria significantly.

Western blot

HEYL Antibody western blot of MDA-MB231 cell line lysates (35 ug/lane). The HEYL antibody detected the HEYL protein (arrow).
HEYL Antibody western blot of MDA-MB231 cell line lysates (35 ug/lane). The HEYL antibody detected the HEYL protein (arrow).

Requested From: 
Date Requested: 5/22/2018

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