HSPA1A antibody LS-C24156 is an AP-conjugated mouse monoclonal antibody to HSPA1A (Heat Shock Protein 70) from human, mouse, rat and other species. Validated for WB.
Human Heat Shock Protein 70 / HSPA1A
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Sheep, Chicken
(tested or 100% immunogen sequence identity)
Protein G purified
Purified Hsp70 isolated from human HeLa cells.
Detects Hsp70 protein (~70kD). Epitope: aa436-503. Does not cross-react with the constitutive Hsc70 (Hsp73). Species cross-reactivity: Human, monkey, rabbit, rat, mouse, hamster, guinea pig, bovine, sheep, canine, chicken, teal, fish (carp), beluga, Drosophila and Xenopus.
Western Blot (Colorimetric): 1:1000. Note: This product may be used directly without the removal of the glycerol. As a blocking solution, we recommend 5% skim milk powder, 0.05% Tween 20 in PBS. This buffer significantly reduces the background observed compared to using only BSA as a blocking agent. Once diluted, the solution should be kept of 4?C and not refrozen. Positive control: Recombinant Human Hsp70 Protein. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
PBS, 0.03% Thimerosal, 50% Glycerol
For research use only.