Human Heat Shock Protein 70 / HSPA1A
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Sheep, Chicken
(tested or 100% immunogen sequence identity)
Protein G purified
Purified Hsp70 isolated from human HeLa cells.
Detects Hsp70 protein (~70kD). Epitope: aa436-503. Does not cross-react with the constitutive Hsc70 (Hsp73). Species cross-reactivity: Human, monkey, rabbit, rat, mouse, hamster, guinea pig, bovine, sheep, canine, chicken, teal, fish (carp), beluga, Drosophila and Xenopus.
Western Blot (Colorimetric): 1:1000. Note: This product may be used directly without the removal of the glycerol. As a blocking solution, we recommend 5% skim milk powder, 0.05% Tween 20 in PBS. This buffer significantly reduces the background observed compared to using only BSA as a blocking agent. Once diluted, the solution should be kept of 4?C and not refrozen. Positive control: Recombinant Human Hsp70 Protein. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
PBS, 0.03% Thimerosal, 50% Glycerol
For research use only.
About Heat Shock Protein 70 / HSPA1A
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins.