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Catalog Number Size Price
LS-B13845-50 50 µl $460 
Heat Shock Protein 70 / HSPA1A Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Heat Shock Protein 70 / HSPA1A Antibody - Immunofluorescence analysis of U2OS cells using HSPA1A antibody. Blue: DAPI for nuclear staining.
Heat Shock Protein 70 / HSPA1A Antibody - Western blot analysis of extracts of various cell lines, using HSPA1A antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
Heat Shock Protein 70 / HSPA1A Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Heat Shock Protein 70 / HSPA1A Antibody - Immunofluorescence analysis of U2OS cells using HSPA1A antibody. Blue: DAPI for nuclear staining.
Heat Shock Protein 70 / HSPA1A Antibody - Western blot analysis of extracts of various cell lines, using HSPA1A antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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IHC‑plus™ Polyclonal Rabbit anti‑Human Heat Shock Protein 70 / HSPA1A Antibody (IHC, IF, WB) LS‑B13845

IHC‑plus™ Polyclonal Rabbit anti‑Human Heat Shock Protein 70 / HSPA1A Antibody (IHC, IF, WB) LS‑B13845

Note: This antibody replaces LS-C330895
Antibody:
Heat Shock Protein 70 / HSPA1A Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B13845-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Heat Shock Protein 70 / HSPA1A Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
HSPA1A antibody LS-B13845 is an unconjugated rabbit polyclonal antibody to HSPA1A (Heat Shock Protein 70) from human. It is reactive with human and mouse. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human Heat Shock Protein 70 / HSPA1A
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide of human HSPA1A
Specificity
Human HSPA1A / HSP72
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 70kDa, while the observed MW by Western blot was 72kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Validation

Heat Shock Protein 70 / HSPA1A Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ Heat Shock Protein 70 / HSPA1A Antibody for IHC, IF/Immunofluorescence, WB/Western LS-B13845 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/28/2024