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Anti-HAUS8 Antibody (phospho-Ser70) LS-C153858

Ordering

Wt. Vol. Conc. Price
100 µg - 0.47 mg/ml $385
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Orders@LSBio.com
 

Most Popular HAUS8 Antibodies

Anti-HAUS8 Antibody (phospho-Ser70) LS-C153858
Rabbit Polyclonal (IgG) to Human HAUS8
Human
Western blot, ELISA
Unconjugated
Western blot Image
Anti-HAUS8 Antibody (aa166-360) LS-C185381
Rabbit Polyclonal (IgG) to Human HAUS8
Human
IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-HAUS8 Antibody IHC-plus™ LS-B10617
Rabbit Polyclonal (IgG) to Human HAUS8
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human HAUS8
Human
Western blot, ELISA
Unconjugated

Details

Human HAUS8
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • Western blot (1.22 µg/ml)
  • ELISA

Specificity and Use

HAUS8 antibody was raised against anti-Hice1 pS70 Antibody was prepared by repeated immunizations with a phosphorylated synthetic peptide corresponding to the region of amino acids containing serine 70 of Hice1.
pSer70
Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. The antibody is specific for the phosphorylated form of Hice1. Reactivity with non-phosphorylated human Hice1 is minimal by ELISA and western blot. A BLAST analysis was used to suggest cross reactivity with Hice1 from human based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 44.9 kD in size corresponding to human phosphorylated Hice1 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About HAUS8

Q9BT25 Q9BT25

HAUS8 Antibody, DGT4 Antibody, NY-SAR-48 Antibody, Sarcoma antigen NY-SAR-48 Antibody, HICE1 Antibody

HAUS8 is 1 of 8 subunits of the 390-kD human augmin complex, or HAUS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb 'augmentare,' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly (Goshima et al., 2008 [PubMed 18443220]; Uehara et al., 2009 [PubMed 19369198]).

Western blot

Western blot
Western blot of Rabbit Anti-Hice1 pS70 antibody. Lane 1: HeLa cell extracts of untransfected cells (-). Lane 2: transfected HeLa cell extracts with Flag X3-Hice1 WT (WT). Lane 3: transfected HeLa cell extracts with Flag X3-Hice1 S70A mutant (70A). Load: 35 ug per lane. Primary antibody: Hice1 pS70 antibody at 0.5 g/mL for overnight at 4C. Secondary antibody: IRDye800 Conjugated Goat Anti-Rabbit IgG secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 44.8 kDa, 48 kDa for Hice1 pS70. Other band(s): none.
 

Requested From: United States
Date Requested: 12/7/2016

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