Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Splicing regulator SC35 is essential for genomic stability and cell proliferation during mammalian organogenesis. Xiao R, Sun Y, Ding JH, Lin S, Rose DW, Rosenfeld MG, Fu XD, Li X. Molecular and cellular biology. 2007 27:5393-402.
Deficiencies in DNA damage repair limit the function of haematopoietic stem cells with age. Rossi DJ, Bryder D, Seita J, Nussenzweig A, Hoeijmakers J, Weissman IL. Nature. 2007 447:725-9.
DNA double-strand breaks, but not crossovers, are required for the reorganization of meiotic nuclei in Tetrahymena. Mochizuki K, Novatchkova M, Loidl J. Journal of cell science. 2008 121:2148-58.
Differential roles of telomere attrition in type I and II endometrial carcinogenesis. Akbay EA, Contreras CM, Perera SA, Sullivan JP, Broaddus RR, Schorge JO, Ashfaq R, Saboorian H, Wong KK, Castrillon DH. The American journal of pathology. 2008 173:536-44.
Tetrahymena meiotic nuclear reorganization is induced by a checkpoint kinase-dependent response to DNA damage. Loidl J, Mochizuki K. Molecular biology of the cell. 2009 20:2428-37.
H2AFX Antibody, H2AX Antibody, Histone H2A.x Antibody, H2A.X Antibody, H2A histone family, member X Antibody, H2AX histone Antibody
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Untreated Hela cells (Panel A), or overnight nocodazole treated Hela cells (Panel B) stained with purified mouse monoclonal antibody against Ser139 phosphorylated H2A.X, followed by Rhodamine Red-X conjugated Donkey anti-mouse IgG and DAPI.
Untreated (Lane 1) and staurosporine-treated (Lane 2) Jurkat nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-H2A. X (Ser 139) antibody. Proteins were visualized using a goat anti-mouse secondary a.