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Anti-H2AFX / H2AX Antibody (phospho-Ser139, clone 2F3) LS-C41114

Note: This antibody replaces LS-C24262

Ordering

Wt. Vol. Conc. Price
100 µg - 0.5 mg/ml $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular H2AFX / H2AX Antibodies

Anti-H2AFX / H2AX Antibody (Ser139) IHC-plus™ LS-B1474
Rabbit Polyclonal (IgG) to Human H2AFX / H2AX
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image
Anti-H2AFX / H2AX Antibody (phospho-Ser139, clone 20E3) LS-C348689
Rabbit Monoclonal [clone 20E3] to Human H2AFX / H2AX
Human, Monkey, Mouse, Rat
IHC, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

100% Guaranteed 100% Guaranteed Publications Publications (5)
Mouse Monoclonal [clone 2F3] (IgG2b) to Human H2AFX / H2AX
Human
Immunofluorescence, Western blot
Unconjugated

Details

Human H2AFX / H2AX
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2b Monoclonal [2F3]
Unconjugated
Affinity purified
Unmodified

Applications

  • Immunofluorescence
  • Western blot

Specificity and Use

H2AFX / H2AX antibody was raised against synthetic peptide from human H2AFX / H2AX.
pSer139
Modified peptide (KATQAS*QEY)

Packaging

Phosphate-buffered solution, pH 7.2, 0.09% sodium azide.
+4°C. Store undiluted.
For research use only.

Publications (5)

1
Splicing regulator SC35 is essential for genomic stability and cell proliferation during mammalian organogenesis. Xiao R, Sun Y, Ding JH, Lin S, Rose DW, Rosenfeld MG, Fu XD, Li X. Molecular and cellular biology. 2007 27:5393-402. [PubMed:17526736] [PMC:PMC1952092]
2
Deficiencies in DNA damage repair limit the function of haematopoietic stem cells with age. Rossi DJ, Bryder D, Seita J, Nussenzweig A, Hoeijmakers J, Weissman IL. Nature. 2007 447:725-9. [PubMed:17554309]
3
DNA double-strand breaks, but not crossovers, are required for the reorganization of meiotic nuclei in Tetrahymena. Mochizuki K, Novatchkova M, Loidl J. Journal of cell science. 2008 121:2148-58. [PubMed:18522989] [PMC:PMC3184542]
4
Differential roles of telomere attrition in type I and II endometrial carcinogenesis. Akbay EA, Contreras CM, Perera SA, Sullivan JP, Broaddus RR, Schorge JO, Ashfaq R, Saboorian H, Wong KK, Castrillon DH. The American journal of pathology. 2008 173:536-44. [PubMed:18599611] [PMC:PMC2475790]
5
Tetrahymena meiotic nuclear reorganization is induced by a checkpoint kinase-dependent response to DNA damage. Loidl J, Mochizuki K. Molecular biology of the cell. 2009 20:2428-37. [PubMed:19297526] [PMC:PMC2675622]
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About H2AFX / H2AX

P16104 NM_002105 NP_002096.1

H2AFX Antibody, H2AX Antibody, Histone H2A.x Antibody, H2A.X Antibody, H2A histone family, member X Antibody, H2AX histone Antibody

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Immunocytochemistry

Immunocytochemistry
Untreated Hela cells (Panel A), or overnight nocodazole treated Hela cells (Panel B) stained with purified mouse monoclonal antibody against Ser139 phosphorylated H2A.X, followed by Rhodamine Red-X conjugated Donkey anti-mouse IgG and DAPI.

Western blot

Western blot
Untreated (Lane 1) and staurosporine-treated (Lane 2) Jurkat nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-H2A. X (Ser 139) antibody. Proteins were visualized using a goat anti-mouse secondary a.

Requested From: United States
Date Requested: 12/6/2016

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