Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
gamma-H2AX foci as a biomarker for patient X-ray exposure in pediatric cardiac catheterization: are we underestimating radiation risks?. Beels L, Bacher K, De Wolf D, Werbrouck J, Thierens H. Circulation. 2009 120:1903-9.
Reduced NR4A gene dosage leads to mixed myelodysplastic/myeloproliferative neoplasms in mice. Ramirez-Herrick AM, Mullican SE, Sheehan AM, Conneely OM. Blood. 2011 117:2681-90.
The SET2-RPB1 interaction domain of human RECQ5 is important for transcription-associated genome stability. Li M, Xu X, Liu Y. Molecular and cellular biology. 2011 31:2090-9.
Variant repeats are interspersed throughout the telomeres and recruit nuclear receptors in ALT cells. Conomos D, Stutz MD, Hills M, Neumann AA, Bryan TM, Reddel RR, Pickett HA. The Journal of cell biology. 2012 199:893-906. (ICC; Human)
Ionizing Radiation-Induced -H2AX Activity in Whole Blood Culture and the Risk of Lung Cancer. He Y, Gong Y, Lin J, Chang DW, Gu J, Roth JA, Wu X. Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology. 2013 22:443-51.
H2AFX Antibody, H2AX Antibody, Histone H2A.x Antibody, H2A.X Antibody, H2A histone family, member X Antibody, H2AX histone Antibody
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Hela cells were fixed in 4% paraformaldehyde, washed in PBS, and permeabilized using 0.5% Triton X-100 for 5 minutes. Cells were then incubated with a 1:50 dilution of H2A. X antibody (Poly6133) for 1 hr, washed twice using 0.1% Triton X-100 in PBS and in.
Jurkat nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-H2A. X antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.