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Anti-GTF2I / TFII I Antibody (aa281-310) LS-C159799

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular GTF2I / TFII I Antibodies

Anti-GTF2I / TFII I Antibody (aa956-985) IHC-plus™ LS-B11236
Rabbit Polyclonal to Human GTF2I / TFII I
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human GTF2I / TFII I
Human
Immunofluorescence, Western blot
Unconjugated

Details

Human GTF2I / TFII I
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)

Specificity and Use

GTF2I / TFII I antibody was raised against kLH-conjugated synthetic peptide between amino acids 281-310 from N-terminal region of human GTF2I.
LS-E5592 - Lyophilized - 100 µg - $145.00
aa281-310
Human GTF2I / TFII I

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About GTF2I / TFII I

P78347 NM_032999 NP_127492.1

GTF2I Antibody, BAP-135 Antibody, BAP135 Antibody, BTK-associated protein, 135kD Antibody, BTK-associated protein 135 Antibody, DIWS Antibody, IB291 Antibody, SRF-Phox1-interacting protein Antibody, WBSCR6 Antibody, TFII I Antibody, TFII-I Antibody, BTKAP1 Antibody, WBS Antibody, GTFII-I Antibody, SPIN Antibody

Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE).

Immunofluorescence

Immunofluorescence
Fluorescent image of HeLa cell stained with GTF2I Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with GTF2I primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). GTF2I immunoreactivity is localized to Nucleus significantly.

Western blot

Western blot
GTF2I Antibody western blot of 293 cell line lysates (35 ug/lane). The GTF2I antibody detected the GTF2I protein (arrow).

Requested From: United States
Date Requested: 12/7/2016

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