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GRM5 / MGLUR5 Antibody (N‑Terminus) IHC‑plus™ LS‑A883
MGLUR5 antibody LS-A883 is an unconjugated rabbit polyclonal antibody to MGLUR5 (GRM5) from human, mouse, rat and other species. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
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Species:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Monkey
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Rat, Mouse
Applications:
IHC, ICC, Immunofluorescence, Western blot
Species:
Human
Applications:
Western blot, Flow Cytometry, ELISA
Product Description
MGLUR5 antibody LS-A883 is an unconjugated rabbit polyclonal antibody to MGLUR5 (GRM5) from human, mouse, rat and other species. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
About GRM5 / MGLUR5
Group I of the Metabotropic Glutamate Receptors includes GRM1 and GRM5, receptors that have been shown to activate phospholipase C. Alternative splice variants of GRM5 have been described, but their full-length nature has not been determined. Inflammation results in the activation of mGluR1 and mGluR5 in dorsal horn neurons of the spinal cord, leading to extracellular signal-regulated kinase (ERK) activation, which is required for nociceptive plasticity and enhanced pain.
P41594
NM_000842
NP_000833.1
GRM5 Antibody, GPRC1E Antibody, MGLUR5 Antibody, Mglur5b Antibody, MGlu5 Antibody
Specifications
Target
Human GRM5 / MGLUR5
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken
(tested or 100% immunogen sequence identity)
Predicted
Xenopus (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
- IHC
- IHC - Paraffin (5.6 µg/ml)
- (applications tested for the base form of this product only)
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Immunogen
GRM5 / MGLUR5 antibody was raised against synthetic 17 amino acid peptide from N-terminal extracellular domain of human GRM5 / MGLUR5. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bat, Bovine, Hamster, Horse, Rabbit, Pig, Turkey, Zebra finch, Chicken, Platypus, Lizard (100%); Xenopus (94%); Opossum, Pufferfish (88%); Zebrafish (82%).
Blocking Peptide
LS-E29491 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A883. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Immunizing peptide used to generate LS-A883. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
Human GRM5 / MGLUR5. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Usage
Immunohistochemistry: LS-A883 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A883 was determined to be 5.6 ug/ml.
Presentation
PBS, less than 0.1% sodium azide.
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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Images
Immunohistochemistry
Anti-GRM5 / MGLUR5 antibody LS-A883 IHC of human colon, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Immunohistochemistry
Anti-GRM5 / MGLUR5 antibody LS-A883 IHC of human colon, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Immunohistochemistry
Anti-GRM5 / MGLUR5 antibody LS-A883 IHC of human colon, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Immunohistochemistry
Anti-GRM5 / MGLUR5 antibody LS-A883 IHC of human colon, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Requested From:
Date Requested: 6/23/2018
Date Requested: 6/23/2018
