MGLUR5 antibody LS-C16546 is an unconjugated rabbit polyclonal antibody to MGLUR5 (GRM5) from mouse and rat. Validated for ICC, IF, IHC and WB.
(tested or 100% immunogen sequence identity)
- IHC (0.1 - 0.2 µg/ml)
- ICC (0.5 µg/ml)
- Western blot (0.1 - 0.5 µg/ml)
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GRM5 / MGLUR5 antibody was raised against synthetic peptide corresponding to rat mGluR5 (Metabotropic Glutamate Receptor).
Recognizes rat metabotropic glutamate receptor 5 (mGluR5). Demonstrates very little or no cross-reactivity with mGluR1a. Species cross-reactivity: rat and mouse.
Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Immunofluorescence. Blocking buffer was removed. Slides were rinsed 3 times. Slides were incubated with secondary antibodies for 1 hour at RT. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy. Immunocytochemistry: 0.5 ug/ml. Immunofluorescence: Indirect.
PBS, pH 7.6, 0.1% sodium azide, 40% glycerol.
Short-term: 4°C, Long-term: Add sterile 40-50% glycerol, aliquot, and store at -20°C.
For research use only.