Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(tested or 100% immunogen sequence identity)
IHC (0.1 - 0.2 µg/ml)
ICC (0.5 µg/ml)
Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
GRM5 / MGLUR5 antibody was raised against synthetic peptide corresponding to rat mGluR5 (Metabotropic Glutamate Receptor).
Recognizes rat metabotropic glutamate receptor 5 (mGluR5). Demonstrates very little or no cross-reactivity with mGluR1a. Species cross-reactivity: rat and mouse.
Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Immunofluorescence. Blocking buffer was removed. Slides were rinsed 3 times. Slides were incubated with secondary antibodies for 1 hour at RT. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy. Immunocytochemistry: 0.5 ug/ml. Immunofluorescence: Indirect.
PBS, pH 7.6, 0.1% sodium azide, 40% glycerol.
Short-term: 4°C, Long-term: Add sterile 40-50% glycerol, aliquot, and store at -20°C.
Group I of the Metabotropic Glutamate Receptors includes GRM1 and GRM5, receptors that have been shown to activate phospholipase C. Alternative splice variants of GRM5 have been described, but their full-length nature has not been determined. Inflammation results in the activation of mGluR1 and mGluR5 in dorsal horn neurons of the spinal cord, leading to extracellular signal-regulated kinase (ERK) activation, which is required for nociceptive plasticity and enhanced pain.