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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GRB14 Antibody (N‑Terminus) LS‑C7298 Best Seller
GRB14 antibody LS-C7298 is an unconjugated rabbit polyclonal antibody to GRB14 from mouse and rat. Validated for ELISA and WB.
Catalog
Size
Price
LS-C7298-100
100 µl
$725
Description
GRB14 antibody LS-C7298 is an unconjugated rabbit polyclonal antibody to GRB14 from mouse and rat. Validated for ELISA and WB.
Target
Rat GRB14
Host
Rabbit
Reactivity
Rat, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Applications
  • Western blot (1:1000 - 1:5000)
  • ELISA (1:10000 - 1:50000)
Immunogen
GRB14 antibody was raised against a 16-aa Peptide ~ within the N-terminus of rat Grb14 (1) was synthesized, coupled to KLH.
Epitope
N-Terminus
Specificity
The control peptide sequence is 100% conserved in mouse and 87% in human Grb14. No significant sequence homology exist with other Grb7 family members. Antibody cross-reactivity in various species is not established.
Usage
Suitable for use in ELISA and Western Blot.
Presentation
PBS, 0.05% Sodium Azide
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About GRB14
Q14449 NM_004490 NP_004481.2

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Immunohistochemistry image at a dilution of 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, ELISA
Immunohistochemical analysis of GRB14 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Antibody
Species: Human
Applications: IHC, Western blot, ELISA
Western blot of GRB14 antibody
Species: Human, Mouse, Rat
Applications: IHC, Western blot, ELISA

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Requested From: United States
Date Requested: 11/20/2018
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