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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GPR49 / LGR5 Antibody (Cytoplasmic Domain) IHC‑plus™ LS‑A1236
LGR5 antibody LS-A1236 is an unconjugated rabbit polyclonal antibody to human LGR5 (GPR49). Validated for IF and IHC. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
Catalog
Size
Price
LS-A1236-50
50 µg (1 mg/ml)
Unavailable
In Stock for Immediate Shipment

Popular GPR49 / LGR5 Products

Fluorescent confocal image of HeLa cells stained with LGR5 (loop2) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated LGR5 (loop2) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the LGR5 immunosignal to the nucleus and cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000139292).
Species: Human
Applications: Immunofluorescence, Western blot, Flow Cytometry
GPR49 Antibody (LGR5) - IHC of human small intestine using GPR49.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Human, Pancreas, Islets: Formalin-Fixed Paraffin-Embedded (FFPE)
Species: Human, Monkey, Bat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence
Human, Brain, neurons: Formalin-Fixed Paraffin-Embedded (FFPE)
Species: Human
Applications: IHC, IHC - Paraffin

Product Description

LGR5 antibody LS-A1236 is an unconjugated rabbit polyclonal antibody to human LGR5 (GPR49). Validated for IF and IHC. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
About GPR49 / LGR5
Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. O75473 NM_003667 NP_003658.1

LGR5 Antibody, FEX Antibody, G-protein coupled receptor 49 Antibody, G protein-coupled receptor 49 Antibody, GPR67 Antibody, GRP49 Antibody, GPR49 Antibody, HG38 Antibody, G-protein coupled receptor 67 Antibody


Specifications

Target
Human GPR49 / LGR5
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey, Bat, Bovine, Hamster, Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
GPR49 / LGR5 antibody was raised against synthetic 20 amino acid peptide from 1st cytoplasmic domain of human GPR49 / LGR5. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey (100%); Marmoset, Bovine, Bat (95%); Hamster, Elephant, Panda, Rabbit (90%); Mouse, Rat, Opossum (85%).
Blocking Peptide
LS-E28194 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A1236. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Cytoplasmic Domain
Specificity
Human GPR49 / LGR5. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Presentation
PBS, 10% glycerol, 0.09% sodium azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (3)

1
Expression of the adult intestinal stem cell marker Lgr5 in the metastatic cascade of colorectal cancer. Kleist B, Xu L, Li G, Kersten C. International journal of clinical and experimental pathology. 2011 4:327-35. (IHC-P; Human) [Full Text Article] [PubMed:21577318] [PMC:PMC3093057]
2
EP receptor expression in human intestinal epithelium and localization relative to the stem cell zone of the crypts. Olsen Hult LT, Kleiveland CR, Fosnes K, Jacobsen M, Lea T. PloS one. 2011 6:e26816. (ICC, IHC-P; Human) [Full Text Article] [PubMed:22046368] [PMC:PMC3201980]
3
Mesenchymal stem cells alleviate TNBS-induced colitis by modulating inflammatory and autoimmune responses. Chen QQ, Yan L, Wang CZ, Wang WH, Shi H, Su BB, Zeng QH, Du HT, Wan J. World journal of gastroenterology : WJG. 2013 Aug;19:4702-17. [Full Text Article] [PubMed:23922467] [PMC:PMC3732842]

Customer Reviews (0)


Images


Immunohistochemistry

Human, Small Intestine: Formalin-Fixed Paraffin-Embedded (FFPE)
Human, Small Intestine: Formalin-Fixed Paraffin-Embedded (FFPE)

Immunohistochemistry

Human Colon: Formalin-Fixed Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed Paraffin-Embedded (FFPE)

Immunohistochemistry

Human, Small Intestine: Formalin-Fixed Paraffin-Embedded (FFPE)
Human, Small Intestine: Formalin-Fixed Paraffin-Embedded (FFPE)

Immunohistochemistry

Human Colon: Formalin-Fixed Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed Paraffin-Embedded (FFPE)

Immunohistochemistry

Human, Small Intestine: Formalin-Fixed Paraffin-Embedded (FFPE)
Human, Small Intestine: Formalin-Fixed Paraffin-Embedded (FFPE)

Immunohistochemistry

Human Colon: Formalin-Fixed Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed Paraffin-Embedded (FFPE)

Immunohistochemistry

Human, Small Intestine: Formalin-Fixed Paraffin-Embedded (FFPE)
Human, Small Intestine: Formalin-Fixed Paraffin-Embedded (FFPE)

Immunohistochemistry

Human Colon: Formalin-Fixed Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed Paraffin-Embedded (FFPE)


Requested From: 
Date Requested: 6/18/2018

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