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Catalog Number Size Price
LS-A1232-50 50 µg (1 mg/ml) $440 
GPR49 / LGR5 Antibody - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
GPR49 / LGR5 Antibody - Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)
GPR49 / LGR5 Antibody - Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)
GPR49 / LGR5 Antibody - Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
GPR49 / LGR5 Antibody - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
GPR49 / LGR5 Antibody - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
GPR49 / LGR5 Antibody - Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)
GPR49 / LGR5 Antibody - Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)
GPR49 / LGR5 Antibody - Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
GPR49 / LGR5 Antibody - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
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PathPlus™ Polyclonal Rabbit anti‑Human GPR49 / LGR5 Antibody (N‑Terminus, IHC) LS‑A1232

PathPlus™ Polyclonal Rabbit anti‑Human GPR49 / LGR5 Antibody (N‑Terminus, IHC) LS‑A1232

Antibody:
GPR49 / LGR5 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC-P
Reactivity:
Human, Monkey, Bat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$440
LS-A1232-50

Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
GPR49 / LGR5 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC-P
Reactivity:
Human, Monkey, Bat
Format:
Unconjugated, Unmodified

Specifications

Description
Leucine-rich repeat containing G protein-coupled receptor 5 (LGR5, GPR49, FEX) is a receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and in hair follicles. Upon binding to R-spondins, such as RSPO1, RSPO2, RSPO3 or RSPO4, it interacts with phosphorylated LRP6 and frizzled receptors and triggers the canonical Wnt signaling pathway to increase expression of target genes. It is involved in the development and maintenance of adult intestinal stem cells during postembryonic development. In immunohistochemistry, it is often used as a marker for LGR5+ multipotent stem cells. It has membranous positivity in various tissues including muscle, the spinal cord, the brain, crypt base columnar cells in the small intestine and in the colon, nascent nephron cell clusters in the developing kidney, populations of cells in the stomach, and the placenta. It is also a useful marker for characterizing stem cells, stem-ness and also poor prognosis in premalignant and cancerous tissues such as glioblastoma, cervical cancer and colorectal carcinoma. References: The UniProt Consortium. Nucleic Acids Res. 47: D506-515 (2019); Nucleic Acids Res. 2016 Jan 4;44(D1):D733-45, PMID:26553804; Gastroenterology. 2010. 138 (5): 1681–96, PMID: 20417836; ScientificWorldJournal. 2008 Nov 23;8:1168-76, PMID: 19030762; Cell Death Dis. 2017 Sep 7;8(9):e3039, PMID: 28880275; Brain Pathol. 2013 Jan;23(1):60-72, PMID: 22805276;
Target
Human GPR49 / LGR5
Synonyms
LGR5 | FEX | G-protein coupled receptor 49 | G protein-coupled receptor 49 | GPR67 | GRP49 | GPR49 | HG38 | G-protein coupled receptor 67
Host
Rabbit
Reactivity
Human, Monkey, Bat (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Bovine (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic 20 amino acid peptide from N-Terminus extracellular domain of human GPR49 / LGR5. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bat (100%); Elephant, Panda (95%); Mouse, Rat, Bovine (90%); Opossum (85%); Rabbit, Chicken (80%).
Epitope
N-Terminus
Specificity
Human GPR49 / LGR5. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Applications
  • IHC - Paraffin (2.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GPR49 / LGR5
O75473 NM_003667 NP_003658.1

Validation

GPR49 / LGR5 Antibody - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)

GPR49 / LGR5 Antibody - Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)

GPR49 / LGR5 Antibody - Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)

GPR49 / LGR5 Antibody - Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)

GPR49 / LGR5 Antibody - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

PathPlus™ GPR49 / LGR5 Antibody (N-Terminus) for IHC LS-A1232 has an LSBio Rating of
Publications (4.4)
Laboratory Validation Score (5)

Publications (5)

EP receptor expression in human intestinal epithelium and localization relative to the stem cell zone of the crypts. Olsen Hult LT, Kleiveland CR, Fosnes K, Jacobsen M, Lea T. PloS one. 2011 6:e26816. (ICC, IHC-P; Human)

Expression of LGR5, an intestinal stem cell marker, during each stage of colorectal tumorigenesis. Takeda K, Kinoshita I, Shimizu Y, Matsuno Y, Shichinohe T, Dosaka-Akita H. Anticancer research. 2011 Jan;31:263-70.

LGR5 is a marker of poor prognosis in glioblastoma and is required for survival of brain cancer stem-like cells. Nakata S, Campos B, Bageritz J, Bermejo JL, Becker N, Engel F, Acker T, Momma S, Herold-Mende C, Lichter P, Radlwimmer B, Goidts V. Brain pathology (Zurich, Switzerland). 2013 Jan;23:60-72.

Immunostaining of Lgr5, an Intestinal Stem Cell Marker, in Normal and Premalignant Human Gastrointestinal Tissue. Becker L, Huang Q, Mashimo H. TheScientificWorldJournal. 2008 November;8:1168-1176. (IHC-P; Human)

LGR5 expression predicts peritoneal recurrence after curative resection of primary colon cancer. Hiroshi Nagata, Soichiro Ishihara, Hiroyuki Abe, Tetsuo Ushiku, Junko Kishikawa, Toshiaki Tanaka, Keisuke Hata, Kazushige Kawai, Masashi Fukayama, Hiroaki Nozawa. British journal of cancer. 2019 May;120:996-1002.

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Customer Reviews (0)


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Requested From: United States
Date Requested: 7/4/2022