Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
IHC - Paraffin (15 - 20 µg/ml), Western blot
Specificity and Use
GPER1 / GPR30 antibody was raised against synthetic 16 amino acid peptide from 1st cytoplasmic domain of human GPR30. Percent identity with other species by BLAST analysis: Human, Gorilla (100%); Marmoset (94%); Monkey, Panda (88%).
Identity of an estrogen membrane receptor coupled to a G protein in human breast cancer cells. Thomas P, Pang Y, Filardo EJ and Dong J. Endocrinology. 2005 146:624-32. (ICC; Human)
The G protein-coupled receptor GPR30 mediates the proliferative effects induced by 17beta-estradiol and hydroxytamoxifen in endometrial cancer cells. Vivacqua A, Bonofiglio D, Recchia AG, Musti AM, Picard D, AndÃ² S and Maggiolini M. Molecular endocrinology (Baltimore, Md.). 2006 20:631-46. (WB; Human)
Inhibition of the AKT/mTOR and erbB pathways by gefitinib, perifosine and analogs of gonadotropin-releasing hormoneI andII to overcome tamoxifen resistance in breast cancer cells. Block M, Gr[Character fc]ndker C, Fister S, Kubin J, Wilkens L, Mueller MD, Hemmerlein B, Emons G, G[Character fc]nthert AR. International journal of oncology. 2012 Nov;41:1845-54. [Full Text Article]
G-protein coupled estrogen receptor that binds to 17-beta-estradiol (E2) with high affinity, leading to rapid and transient activation of numerous intracellular signaling pathways. Stimulates cAMP production, calcium mobilization and tyrosine kinase Src inducing the release of heparin-bound epidermal growth factor (HB-EGF) and subsequent transactivation of the epidermal growth factor receptor (EGFR), activating downstream signaling pathways such as PI3K/Akt and ERK/MAPK.