Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Monkey, Dog (tested or 100% immunogen sequence identity)
Mouse, Rat (at least 90% immunogen sequence identity)
IHC - Paraffin (3.75 - 7.5 µg/ml)
Specificity and Use
GPER1 / GPR30 antibody was raised against synthetic peptide C-KAVIPDSTEQSDVR from positions 357-370 of human GPER (NP_001496.1). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Dog (100%); Mouse, Rat (93%); Hamster, Horse (86%).
G-protein coupled estrogen receptor that binds to 17-beta-estradiol (E2) with high affinity, leading to rapid and transient activation of numerous intracellular signaling pathways. Stimulates cAMP production, calcium mobilization and tyrosine kinase Src inducing the release of heparin-bound epidermal growth factor (HB-EGF) and subsequent transactivation of the epidermal growth factor receptor (EGFR), activating downstream signaling pathways such as PI3K/Akt and ERK/MAPK.