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Anti-GNAO1 Antibody (aa345-354) IHC-plus™ LS-B3655

Note: This antibody replaces LS-C41953

Ordering

Wt. Vol. Conc. Price
- 250 µl 5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular GNAO1 Antibodies

Anti-GNAO1 Antibody (aa345-354) IHC-plus™ LS-B3655
Rabbit Polyclonal (IgG) to Mouse GNAO1
Mouse, Drosophila
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-GNAO1 Antibody (aa108-157) IHC-plus™ LS-B6634
Rabbit Polyclonal (IgG) to Human GNAO1
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Rabbit, Xenopus
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-GNAO1 Antibody (clone 2A) LS-C152924
Mouse Monoclonal [clone 2A] (IgG1) to Bovine GNAO1
Bovine, Human, Mouse, Rat, Guinea pig
Western blot
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Mouse GNAO1
Mouse, Drosophila
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Mouse GNAO1
Rabbit
Mouse, Drosophila (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot
  • ELISA (1:500 - 1:1000)

Specificity and Use

GNAO1 antibody was raised against synthetic peptide from mouse GNAO1 / GNAO.
aa345-354
Recognizes the alpha subunit of murine Guanine nucleotide-binding protein G(o). Guanine nucleotide-binding protein G(o) is a 354 amino acid membrane associated protein of ~40kD forming a heterotrimeric protein complex with beta and gamma subunits. Two isoforms of the alpha subunit are generated by alternative splicing with a single amino acid substitution in the immunogen sequence (a lysine for an asparagine at position 346), it is not known whether this substitution affects the binding to isoform 2. Has been used successfully for the detection of G(o) in Drosophila by western blotting.
Immunohistochemistry: LS-B3655 was validated for use in immunohistochemistry on a panel of formalin-fixed, paraffin-embedded (FFPE) mouse tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3655 was determined to be 10 ug/ml. Western Blot: This antibody detects band at 39 kD when used under reducing conditions against rat brain.

Packaging

PBS, 0.09% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About GNAO1

P09471 NM_020988 NP_066268.1

GNAO1 Antibody, GNAO Antibody, G-ALPHA-o Antibody, G0 Protein Alpha Antibody

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(o) protein function is not clear. Stimulated by RGS14.

Immunohistochemistry

Immunohistochemistry
Anti-GNAO1 antibody IHC of mouse brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3655 concentration 10 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-GNAO1 antibody IHC of mouse brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3655 concentration 10 ug/ml.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Synthetic G Protein Alpha 0 detected with Rabbit anti-Mouse G Protein Alpha 0 (LS-B3655).
 

Requested From: United States
Date Requested: 12/3/2016

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