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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GFAP Antibody (clone SPM507, Azide‑free) LS‑C392251
GFAP antibody LS-C392251 is an unconjugated mouse monoclonal antibody to GFAP from human, mouse, rat and other species. Validated for Flow, IF, IHC and WB.
Catalog
Size
Price
LS-C392251-100
100 µg (1 mg/ml)
Unavailable

Popular GFAP Products

Anti-GFAP antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B285 dilution 1:500.
Species: Pig, Human, Mouse, Rat, Bovine, Cat, Dog, Guinea pig, Rabbit, Chicken
Applications: IHC, IHC - Paraffin
Anti-GFAP antibody IHC of human brain, cortex, astrocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2192 concentration 5 ug/ml.
Species: Human, Monkey, Rat, Bat, Bovine, Horse, Rabbit
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-GFAP antibody IHC of human cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4775 dilution 1:100.
Species: Bovine, Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Western blot
Anti-GFAP antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5176 dilution 1:50.
Species: Bovine, Human, Monkey, Mouse, Rat, Guinea pig, Hamster, Sheep, Chicken
Applications: IHC, IHC - Paraffin
Anti-GFAP antibody IHC of human brain, astrocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6299 dilution 1:100.
Species: Mouse, Human
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot

Product Description

GFAP antibody LS-C392251 is an unconjugated mouse monoclonal antibody to GFAP from human, mouse, rat and other species. Validated for Flow, IF, IHC and WB.
About GFAP
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. P14136 NM_002055 NP_002046.1

GFAP Antibody


Specifications

Target
Pig GFAP
Host
Mouse
Reactivity
Pig, Human, Mouse, Rat, Bovine, Rabbit, Chicken (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [SPM507]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Azide-free. Also available Unmodified or Ready-to-Use.
Applications
  • IHC
  • IHC - Paraffin (0.25 - 0.5 µg/ml)
  • Immunofluorescence (1 - 2 µg/ml)
  • Western blot (0.5 - 1 µg/ml)
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
GFAP antibody was raised against gFAP isolated from pig spinal cord.
Specificity
Pig GFAP
Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. This mAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS. The optimal dilution of the GFAP antibody for each application should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Presentation
Sterile PBS.
Storage
Store at 2°C to 8°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Immunohistochemistry

Formalin-fixed, paraffin-embedded human cerebellum stained with GFAP antibody (SPM507).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human cerebellum stained with GFAP antibody (SPM507). This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human cerebellum stained with GFAP antibody (SPM507).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human cerebellum stained with GFAP antibody (SPM507). This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human cerebellum stained with GFAP antibody (SPM507).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human cerebellum stained with GFAP antibody (SPM507). This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human cerebellum stained with GFAP antibody (SPM507).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human cerebellum stained with GFAP antibody (SPM507). This image was taken for the unmodified form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 7/18/2018

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