Pig, Human, Mouse, Rat, Bovine, Rabbit, Chicken
(tested or 100% immunogen sequence identity)
Protein G purified
Also available Unmodified
- IHC - Paraffin (0.25 - 0.5 µg/ml)
- Immunofluorescence (1 - 2 µg/ml)
- Western blot (0.5 - 1 µg/ml)
- Flow Cytometry (0.5 - 1 µg/10E6 cells)
- (applications tested for the base form of this product only)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
GFAP antibody was raised against gFAP isolated from pig spinal cord.
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. This mAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS. The optimal dilution of the GFAP antibody for each application should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Store at 2°C to 8°C.
For research use only.
This antibody carries the LSBio 100% Guarantee.