Pig, Human, Mouse, Rat, Bovine, Rabbit, Chicken
(tested or 100% immunogen sequence identity)
[GA-5 + ASTRO/789]
Protein G purified
Also available Unmodified
- IHC - Paraffin (1:1)
- Western blot
- Flow Cytometry
- (applications tested for the base form of this product only)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
GFAP antibody was raised against gFAP isolated from pig spinal cord (GA-5) and recombinant protein (ASTRO/789) were used as the immunogen for the GFAP antibody cocktail.
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. This mAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS. Optimal dilution of the GFAP antibody cocktail should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
PBS, 0.01% BSA, 0.05% sodium azide
Store at 2°C to 8°C.
For research use only.