Monoclonal Mouse anti‑Pig GFAP Antibody (clone GA‑5 + ASTRO/789, Azide‑free,BSA‑free, IHC, IF, WB) LS‑C390221
Monoclonal Mouse anti‑Pig GFAP Antibody (clone GA‑5 + ASTRO/789, Azide‑free,BSA‑free, IHC, IF, WB) LS‑C390221
Antibody:
GFAP Mouse anti-Pig Monoclonal (Azide-free, BSA-free) (GA-5 + ASTRO/789) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Pig, Human, Mouse, Rat, Bovine, Rabbit, Chicken
Format:
Unconjugated, Azide-free, BSA-free
Overview
Antibody:
GFAP Mouse anti-Pig Monoclonal (Azide-free, BSA-free) (GA-5 + ASTRO/789) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Pig, Human, Mouse, Rat, Bovine, Rabbit, Chicken
Format:
Unconjugated, Azide-free, BSA-free
Specifications
Description
GFAP antibody LS-C390221 is an unconjugated mouse monoclonal antibody to GFAP from pig. It is reactive with human, mouse, rat and other species. Validated for Flow, IF, IHC and WB.
Reactivity
Pig, Human, Mouse, Rat, Bovine, Rabbit, Chicken
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Azide-free, BSA-free.
Also available
Unmodified or
Ready-to-Use.
Immunogen
GFAP isolated from pig spinal cord (GA-5) and recombinant protein (ASTRO/789) were used as the immunogen for the GFAP antibody cocktail.
Applications
- IHC
- IHC - Paraffin (0.25 - 0.5 µg/ml)
- Immunofluorescence (1 - 2 µg/ml)
- Western blot (0.5 - 1 µg/ml)
- Flow Cytometry (0.5 - 1 µg/10E6 cells)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. This mAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS. Optimal dilution of the GFAP antibody cocktail should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Storage
Store at 2°C to 8°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
Featured Products
Species:
Human, Monkey, Rat, Bat, Bovine, Horse, Rabbit
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Pig, Human, Mouse, Rat, Bovine, Cat, Dog, Guinea pig, Rabbit, Chicken
Applications:
IHC, IHC - Paraffin
Species:
Bovine, Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, ICC, Western blot
Species:
Human, Rat, Porcine
Applications:
IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Species:
Bovine, Human, Rat, Dog, Mammal
Applications:
IHC, ICC
Species:
Human, Mouse, Rat
Applications:
Immunoprecipitation
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 9/19/2024