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Catalog Number	Size	Price
LS-C343953-10	10 µg	$318
LS-C343953-100	100 µg (0.5 mg/ml)	$470

GFAP Antibody - GFAP antibody IHC-paraffin: Human meningioma Tissue.

GFAP Antibody - IHC analysis of GFAP using anti-GFAP antibody. GFAP was detected in paraffin-embedded section of Human meningioma Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-GFAP Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

GFAP Antibody - GFAP antibody IHC-paraffin: Mouse Brain Tissue.

GFAP Antibody - GFAP antibody IHC-paraffin: Rat Brain Tissue.

GFAP Antibody - IF analysis of GFAP using anti-GFAP antibody. GFAP was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-GFAP Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

GFAP Antibody - Western blot analysis of GFAP using anti-GFAP antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate Lane 2: Mouse Brain Tissue Lysate Lane 3: U87 Whole Cell Lysate Lane 4: SHG Whole Cell Lysate Lane 5: NEURO Whole Cell Lysate Lane 6: Hela Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GFAP antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GFAP at approximately 49KD. The expected band size for GFAP is at 49KD.

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Polyclonal Rabbit anti‑Human GFAP Antibody (aa93‑432, IHC, WB) LS‑C343953

Name: Anti-GFAP Antibody | Rabbit anti-Human Polyclonal IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C343953-10
Price: 318.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

GFAP Rabbit anti-Human Polyclonal (aa93-432) Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

$318

LS-C343953-10

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

GFAP Rabbit anti-Human Polyclonal (aa93-432) Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

GFAP antibody LS-C343953 is an unconjugated rabbit polyclonal antibody to GFAP (aa93-432) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.

Target

Human GFAP

Synonyms

GFAP

See All GFAP Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

E.coli-derived human GFAP recombinant protein (Position: Q93-M432). Human GFAP shares 94% amino acid (aa) sequence identity with both mouse and rat GFAP.

Epitope

aa93-432

Specificity

Expressed in cells lacking fibronectin. .

Applications

IHC
IHC - Paraffin
Western blot

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About GFAP

Uniprot: P14136 NCBI: NM_002055 NP_002046.1