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Anti-GCLC Antibody (clone 3H1) LS-C197304

Ordering

Wt. Vol. Conc. Price
100 µg - - $355
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular GCLC Antibodies

Anti-GCLC Antibody (aa295-313) IHC-plus™ LS-B2328
Rabbit Polyclonal to Rat GCLC
Rat, Human, Monkey, Mouse, Bovine
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-GCLC Antibody (aa323-372) IHC-plus™ LS-B6899
Rabbit Polyclonal to Human GCLC
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish, Drosophila
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-GCLC Antibody (aa323-372, FITC) LS-C458773
Rabbit Polyclonal to Human GCLC
Human, Chimpanzee, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish, Drosophila
IHC - Paraffin, Western blot
FITC Conjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 3H1] (IgG1,k) to Human GCLC
Human
Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human GCLC
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [3H1]
Unconjugated
Protein A purified
Unmodified

Applications

  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA

Specificity and Use

GCLC antibody was raised against gCLC (NP_001489, aa528-637 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human GCLC
Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.2
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About GCLC

P48506 NM_001498 NP_001489.1

GCLC Antibody, GCL Antibody, GCS heavy chain Antibody, Gamma-ECS Antibody, GCS Antibody, GLCL Antibody, GLCLC Antibody

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21.

Immunofluorescence

Immunofluorescence
Immunofluorescence of monoclonal antibody to GCLC on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western blot
Western Blot analysis of GCLC expression in transfected 293T cell line by GCLC monoclonal antibody (M01), clone 3H1.Lane 1: GCLC transfected lysate(72.8 KDa).Lane 2: Non-transfected lysate.

Western blot

Western blot
GCLC monoclonal antibody (M01), clone 3H1 Western Blot analysis of GCLC expression in A-431.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged GCLC is approximately 0.03 ng/ml as a capture antibody.

Requested From: United States
Date Requested: 12/8/2016

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