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Anti-GAPDH Antibody IHC-plus™ LS-B4075

Note: This antibody replaces LS-C109289

Ordering

Wt. Vol. Conc. Price
- 50 µl 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular GAPDH Antibodies

Anti-GAPDH Antibody (aa30-44, Magnetic beads) LS-C172169
Rabbit Polyclonal to Human GAPDH
Human, Mouse, Rat
IHC - Paraffin, IHC - Frozen, ICC, Western blot, Immunoprecipitation
Magnetic beads Conjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human GAPDH
Human, Mouse, Rat, Drosophila
IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated

Details

Human GAPDH
Rabbit
Human, Mouse, Rat, Drosophila (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • ICC (1:100 - 1:200)
  • Immunofluorescence (1:100 - 1:200)
  • Western blot (1:5000 - 1:20000)

Specificity and Use

GAPDH antibody was raised against recombinant protein fragment containing a sequence corresponding to a region within amino acids 9 and 323 of GAPDH.
Human GAPDH
Cross-reactiviy with mouse and zebrafish has been confirmed in WB only. The antibody's suitability for use in IHC with mouse and zebrafish tissues has not been determined at this time. Cross-reactivity with rat, rabbit, cow, dog, and cat has not been experimentally confirmed at this time. The antibody is predicted to cross react with these species based on the following homology: Rat (94%), Rabbit (94%), cow (94%), Dog (95%), Cat (96%).

Packaging

PBS, 20% glycerol (pH 7), 0.025% proclin
Aliquot and store at -20°C. Minimize freezing and thawing.
For research use only.

About GAPDH

P04406 NM_002046 NP_002037.2

GAPDH Antibody, A1 40 kd subunit Antibody, Activator 1 40 kd subunit Antibody, G3PD Antibody, GAPD Antibody, G3pdh Antibody, Rfc40 Antibody, Rf-c 40 kd subunit Antibody

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton.

Immunohistochemistry

Immunohistochemistry
Anti-GAPDH antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4075 concentration 10 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-GAPDH antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4075 concentration 10 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of paraformaldehyde-fixed A549, using GAPDH antibody at 1:200 dilution.

Western blot

Western blot
Sample (50 ug of whole cell lysate) A: Mouse Brain. 10% SDS PAGE. LS-B4075 diluted at 1:10000.

Western blot

Western blot
Sample (30 ug of whole cell lysate). A: 293T. B: A431. C: H1299. D: Hela. 10% SDS PAGE. GAPDH antibody diluted at 1:5000.
 

Requested From: United States
Date Requested: 12/9/2016

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