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Catalog Number Size Price
LS-B2536-50 50 µg (1 mg/ml) $440 
GAPDH Antibody - Anti-GAPDH antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
GAPDH Antibody - Western blot of GAPDH in the multiple human tumor cell line lysate INSTA-Blot using antibody at 0.25 ug/ml.
GAPDH Antibody - Western blot of GAPDH using antibody at 1:500in nuclear and cytoplasmic fractions made from Drosophila head extracts.
GAPDH Antibody - Anti-GAPDH antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
GAPDH Antibody - Western blot of GAPDH in the multiple human tumor cell line lysate INSTA-Blot using antibody at 0.25 ug/ml.
GAPDH Antibody - Western blot of GAPDH using antibody at 1:500in nuclear and cytoplasmic fractions made from Drosophila head extracts.
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IHC‑plus™ Polyclonal Rabbit anti‑Human GAPDH Antibody (aa73‑87, IHC, WB) LS‑B2536

IHC‑plus™ Polyclonal Rabbit anti‑Human GAPDH Antibody (aa73‑87, IHC, WB) LS‑B2536

Note: This antibody replaces LS-C19379, LS-C66761
Antibody:
GAPDH Rabbit anti-Human Polyclonal (aa73-87) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Price
Catalog Number
$440
LS-B2536-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
GAPDH Rabbit anti-Human Polyclonal (aa73-87) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified

Specifications

Description
GAPDH antibody LS-B2536 is an unconjugated rabbit polyclonal antibody to GAPDH (aa73-87) from human. It is reactive with human and monkey. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human GAPDH
Synonyms
GAPDH | A1 40 kd subunit | Activator 1 40 kd subunit | G3PD | GAPD | G3pdh | Rfc40 | Rf-c 40 kd subunit
Host
Rabbit
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Predicted
Pig (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Amino acids 73-87 (PITIFQERDPSKIKW) of glyceraldehyde 3-phosphate dehydrogenase protein were used as the immunogen. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset (100%); Pig (93%); Mouse, Rat, Cat, Chicken, Armadillo, Xenopus (87%).
Epitope
aa73-87
Specificity
Amino acids 73-87 (PITIFQERDPSKIKW) of glyceraldehyde 3-phosphate dehydrogenase protein was used as the immunogen.
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B2536 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2536 was determined to be 5 ug/ml.
Presentation
PBS, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GAPDH
P04406 NM_002046 NP_002037.2

Validation

GAPDH Antibody - Anti-GAPDH antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-GAPDH antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-GAPDH antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ GAPDH Antibody (aa73-87) for IHC, WB/Western LS-B2536 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/27/2024