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Anti-GAPDH Antibody (HRP) IHC-plus™ LS-B2193

Note: This antibody replaces LS-C51085

Ordering

Wt. Vol. Conc. Price
100 µg - 0.5 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

GAPDH Antibody LS-B2193 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-GAPDH antibody IHC of human breast.
Western blot
Western blot of cell lysates and tissue lysates using GAPDH Antibody [HRP], pAb, Goat (LS-B2193,...
Western blot
Western blot of cell lysates using GAPDH Antibody [HRP], pAb, Goat (LS-B2193, 1 ug/ml) The...
Western blot
Western blot of cell and tissue lysates using GAPDH Antibody [HRP], pAb, Goat (LS-B2193, 0.2...

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Goat Polyclonal (IgG) to Human GAPDH
Human, Mouse, Rat, Bovine, Goat, Hamster, Rabbit, Chicken, Fish, E. coli, Swine
IHC - Paraffin, Western blot
HRP Conjugated

Details

Human GAPDH
Goat
Human, Mouse, Rat, Bovine, Goat, Hamster, Rabbit, Chicken, Fish, E. coli, Swine (tested or 100% immunogen sequence identity)
IgG Polyclonal
Horseradish Peroxidase (HRP)
Immunoaffinity purified

Applications

IHC - Paraffin (2.5 µg/ml), Western blot

Specificity and Use

GAPDH antibody was raised against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from rabbit muscle.
GAPDH from human, mouse, rat, goat, rabbit, chicken, hamster, bovine, swine, fish, and E. coli cell and tissue lysates. It has not yet been tested with other species.
Immunohistochemistry: LS-B2193 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2193 was determined to be 2.5 ug/ml.

Packaging

PBS, pH 7.4, 1% BSA, 0.01% Thimerosal.
Reconstitute with deionized water to a concentration of 0.5 mg/ml
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About GAPDH

P04406 NM_002046 NP_002037.2

GAPDH Antibody, A1 40 kd subunit Antibody, Activator 1 40 kd subunit Antibody, G3PD Antibody, GAPD Antibody, G3pdh Antibody, Rfc40 Antibody, Rf-c 40 kd subunit Antibody

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton.

Immunohistochemistry

Immunohistochemistry
Anti-GAPDH antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2193 concentration 5 ug/ml.

Western blot

Western blot
Western blot of cell lysates and tissue lysates using GAPDH Antibody [HRP], pAb, Goat (LS-B2193, 0.05 ug/ml) The signal was developed with LumiSensor HRP Substrate Kit Predicted Size: 36 kD Observed Size: 36 kD

Western blot

Western blot
Western blot of cell lysates using GAPDH Antibody [HRP], pAb, Goat (LS-B2193, 1 ug/ml) The signal was developed with LumiSensor HRP Substrate Kit Predicted Size: 36 kD Observed Size: 36 kD

Western blot

Western blot
Western blot of cell and tissue lysates using GAPDH Antibody [HRP], pAb, Goat (LS-B2193, 0.2 ug/ml) The signal was developed with LumiSensor HRP Substrate Kit Predicted Size: 36 kD Observed Size: 36 kD

Requested From: United States
Date Requested: 9/5/2015

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