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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GAPDH Antibody LS‑C210926
GAPDH antibody LS-C210926 is an unconjugated rabbit polyclonal antibody to GAPDH from human, mouse and zebrafish. Validated for ELISA, IHC and WB.
Catalog
Size
Price
LS-C210926-100
100 µg
$385

Popular GAPDH Products

Anti-GAPDH antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2536 concentration 5 ug/ml.
Species: Human, Monkey
Applications: IHC, IHC - Paraffin, Western blot
Anti-GAPDH antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8020 dilution 10 ug/ml.
Species: Human, Mouse, Rat, Bovine, Cat, Dog, Goat, Pig, Rabbit, Fish
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
 This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat
Applications: IHC, Western blot, Immunoprecipitation
Western blot of cell lysates using GAPDH Antibody, pAb, Goat (LS-C51084, 0.1 ug/ml) The signal was developed with Donkey Anti-Goat IgG (H&L) [HRP] Polyclonal Antibody and LumiSensor HRP Substrate Kit. Predicted Size: 36 kD Observed Size: 36 kD
Species: Human, Mouse, Rat, Bovine, Goat, Hamster, Pig, Chicken, Fish, E. coli
Applications: Western blot, ELISA
Anti-GAPDH antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2517 concentration 5 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot

Product Description

GAPDH antibody LS-C210926 is an unconjugated rabbit polyclonal antibody to GAPDH from human, mouse and zebrafish. Validated for ELISA, IHC and WB.
About GAPDH
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. P04406 NM_002046 NP_002037.2

GAPDH Antibody, A1 40 kd subunit Antibody, Activator 1 40 kd subunit Antibody, G3PD Antibody, GAPD Antibody, G3pdh Antibody, Rfc40 Antibody, Rf-c 40 kd subunit Antibody


Specifications

Target
Human GAPDH
Host
Rabbit
Reactivity
Human, Mouse, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Caprylic acid and ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • Western blot
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
GAPDH antibody was raised against recombinant human Glyceraldehyde-3-phosphate dehydrogenase protein.
Specificity
Human GAPDH
Presentation
PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Storage
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Images

Immunohistochemistry

Immunohistochemistry with GADPH antibody at a 1:50 dilution on paraffin-embdeed mouse liver
Immunohistochemistry with GADPH antibody at a 1:50 dilution on paraffin-embdeed mouse liver

Western blot

Western blot using Glyceraldehyde-3-phosphate dehydrogenase antibody at 2.6 ug/ml. Lane 1: HepG2 whole cell lysate, Lane 2: Mouse brain lysate, Lane 3: Mouse skeletal muscle lysate
Western blot using Glyceraldehyde-3-phosphate dehydrogenase antibody at 2.6 ug/ml. Lane 1: HepG2 whole cell lysate, Lane 2: Mouse brain lysate, Lane 3: Mouse skeletal muscle lysate

Western blot

Western blot using GADPH antibody at 2 ug/ml.  Lane 1, HeLa cell lysate; Lane 2, A549 cell lysate; Lane 3, HepG2 cell lysates; Lane 4, K562 cell lysate; Lane 5, Jurkat cell lysate; Lane 6, MDA-MB-231 cell lysate; Lane 7, LO2 cell lysate; Lane 8, U251 cell lysate; and Lane 9, MCF-7 cell lysate.
Western blot using GADPH antibody at 2 ug/ml. Lane 1, HeLa cell lysate; Lane 2, A549 cell lysate; Lane 3, HepG2 cell lysates; Lane 4, K562 cell lysate; Lane 5, Jurkat cell lysate; Lane 6, MDA-MB-231 cell lysate; Lane 7, LO2 cell lysate; Lane 8, U251 cell lysate; and Lane 9, MCF-7 cell lysate.

Western blot

Western blot using GADPH antibody at 2 ug/ml.  Lane 1, HeLa cell lysate; Lane 2, 293T cell lysate; Lane 3, Jurkat cell lysates; Lane 4, Mouse kidney extract; Lane 5, Rat muscle extract; Lane 6, Rat lung extract; and Lane 7, Zebrafish extract.
Western blot using GADPH antibody at 2 ug/ml. Lane 1, HeLa cell lysate; Lane 2, 293T cell lysate; Lane 3, Jurkat cell lysates; Lane 4, Mouse kidney extract; Lane 5, Rat muscle extract; Lane 6, Rat lung extract; and Lane 7, Zebrafish extract.

Immunohistochemistry

Immunohistochemistry with GADPH antibody at a 1:50 dilution on paraffin-embdeed mouse liver
Immunohistochemistry with GADPH antibody at a 1:50 dilution on paraffin-embdeed mouse liver

Western blot

Western blot using Glyceraldehyde-3-phosphate dehydrogenase antibody at 2.6 ug/ml. Lane 1: HepG2 whole cell lysate, Lane 2: Mouse brain lysate, Lane 3: Mouse skeletal muscle lysate
Western blot using Glyceraldehyde-3-phosphate dehydrogenase antibody at 2.6 ug/ml. Lane 1: HepG2 whole cell lysate, Lane 2: Mouse brain lysate, Lane 3: Mouse skeletal muscle lysate

Western blot

Western blot using GADPH antibody at 2 ug/ml.  Lane 1, HeLa cell lysate; Lane 2, A549 cell lysate; Lane 3, HepG2 cell lysates; Lane 4, K562 cell lysate; Lane 5, Jurkat cell lysate; Lane 6, MDA-MB-231 cell lysate; Lane 7, LO2 cell lysate; Lane 8, U251 cell lysate; and Lane 9, MCF-7 cell lysate.
Western blot using GADPH antibody at 2 ug/ml. Lane 1, HeLa cell lysate; Lane 2, A549 cell lysate; Lane 3, HepG2 cell lysates; Lane 4, K562 cell lysate; Lane 5, Jurkat cell lysate; Lane 6, MDA-MB-231 cell lysate; Lane 7, LO2 cell lysate; Lane 8, U251 cell lysate; and Lane 9, MCF-7 cell lysate.

Western blot

Western blot using GADPH antibody at 2 ug/ml.  Lane 1, HeLa cell lysate; Lane 2, 293T cell lysate; Lane 3, Jurkat cell lysates; Lane 4, Mouse kidney extract; Lane 5, Rat muscle extract; Lane 6, Rat lung extract; and Lane 7, Zebrafish extract.
Western blot using GADPH antibody at 2 ug/ml. Lane 1, HeLa cell lysate; Lane 2, 293T cell lysate; Lane 3, Jurkat cell lysates; Lane 4, Mouse kidney extract; Lane 5, Rat muscle extract; Lane 6, Rat lung extract; and Lane 7, Zebrafish extract.

Immunohistochemistry

Immunohistochemistry with GADPH antibody at a 1:50 dilution on paraffin-embdeed mouse liver
Immunohistochemistry with GADPH antibody at a 1:50 dilution on paraffin-embdeed mouse liver

Western blot

Western blot using Glyceraldehyde-3-phosphate dehydrogenase antibody at 2.6 ug/ml. Lane 1: HepG2 whole cell lysate, Lane 2: Mouse brain lysate, Lane 3: Mouse skeletal muscle lysate
Western blot using Glyceraldehyde-3-phosphate dehydrogenase antibody at 2.6 ug/ml. Lane 1: HepG2 whole cell lysate, Lane 2: Mouse brain lysate, Lane 3: Mouse skeletal muscle lysate

Western blot

Western blot using GADPH antibody at 2 ug/ml.  Lane 1, HeLa cell lysate; Lane 2, A549 cell lysate; Lane 3, HepG2 cell lysates; Lane 4, K562 cell lysate; Lane 5, Jurkat cell lysate; Lane 6, MDA-MB-231 cell lysate; Lane 7, LO2 cell lysate; Lane 8, U251 cell lysate; and Lane 9, MCF-7 cell lysate.
Western blot using GADPH antibody at 2 ug/ml. Lane 1, HeLa cell lysate; Lane 2, A549 cell lysate; Lane 3, HepG2 cell lysates; Lane 4, K562 cell lysate; Lane 5, Jurkat cell lysate; Lane 6, MDA-MB-231 cell lysate; Lane 7, LO2 cell lysate; Lane 8, U251 cell lysate; and Lane 9, MCF-7 cell lysate.

Western blot

Western blot using GADPH antibody at 2 ug/ml.  Lane 1, HeLa cell lysate; Lane 2, 293T cell lysate; Lane 3, Jurkat cell lysates; Lane 4, Mouse kidney extract; Lane 5, Rat muscle extract; Lane 6, Rat lung extract; and Lane 7, Zebrafish extract.
Western blot using GADPH antibody at 2 ug/ml. Lane 1, HeLa cell lysate; Lane 2, 293T cell lysate; Lane 3, Jurkat cell lysates; Lane 4, Mouse kidney extract; Lane 5, Rat muscle extract; Lane 6, Rat lung extract; and Lane 7, Zebrafish extract.

Immunohistochemistry

Immunohistochemistry with GADPH antibody at a 1:50 dilution on paraffin-embdeed mouse liver
Immunohistochemistry with GADPH antibody at a 1:50 dilution on paraffin-embdeed mouse liver

Western blot

Western blot using Glyceraldehyde-3-phosphate dehydrogenase antibody at 2.6 ug/ml. Lane 1: HepG2 whole cell lysate, Lane 2: Mouse brain lysate, Lane 3: Mouse skeletal muscle lysate
Western blot using Glyceraldehyde-3-phosphate dehydrogenase antibody at 2.6 ug/ml. Lane 1: HepG2 whole cell lysate, Lane 2: Mouse brain lysate, Lane 3: Mouse skeletal muscle lysate

Western blot

Western blot using GADPH antibody at 2 ug/ml.  Lane 1, HeLa cell lysate; Lane 2, A549 cell lysate; Lane 3, HepG2 cell lysates; Lane 4, K562 cell lysate; Lane 5, Jurkat cell lysate; Lane 6, MDA-MB-231 cell lysate; Lane 7, LO2 cell lysate; Lane 8, U251 cell lysate; and Lane 9, MCF-7 cell lysate.
Western blot using GADPH antibody at 2 ug/ml. Lane 1, HeLa cell lysate; Lane 2, A549 cell lysate; Lane 3, HepG2 cell lysates; Lane 4, K562 cell lysate; Lane 5, Jurkat cell lysate; Lane 6, MDA-MB-231 cell lysate; Lane 7, LO2 cell lysate; Lane 8, U251 cell lysate; and Lane 9, MCF-7 cell lysate.

Western blot

Western blot using GADPH antibody at 2 ug/ml.  Lane 1, HeLa cell lysate; Lane 2, 293T cell lysate; Lane 3, Jurkat cell lysates; Lane 4, Mouse kidney extract; Lane 5, Rat muscle extract; Lane 6, Rat lung extract; and Lane 7, Zebrafish extract.
Western blot using GADPH antibody at 2 ug/ml. Lane 1, HeLa cell lysate; Lane 2, 293T cell lysate; Lane 3, Jurkat cell lysates; Lane 4, Mouse kidney extract; Lane 5, Rat muscle extract; Lane 6, Rat lung extract; and Lane 7, Zebrafish extract.

Requested From: United States
Date Requested: 9/25/2018

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