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Anti-GAPDH Antibody (C-Terminus) IHC-plus™ LS-B9773

Note: This antibody replaces LS-C204214

Ordering

Wt. Vol. Conc. Price
200 µg 100 µl 2 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular GAPDH Antibodies

Anti-GAPDH Antibody (aa30-44, Magnetic beads) LS-C172169
Rabbit Polyclonal to Human GAPDH
Human, Mouse, Rat
IHC - Paraffin, IHC - Frozen, ICC, Western blot, Immunoprecipitation
Magnetic beads Conjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Human GAPDH
Human, Monkey, Mouse, Rat, Dog
IHC - Paraffin, Western blot
Unconjugated

Details

Human GAPDH
Goat
Human, Monkey, Mouse, Rat, Dog (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:500 - 1:2000)

Specificity and Use

GAPDH antibody was raised against purified recombinant peptide derived from within residues 240 aa to the C-terminus of human GAPDH produced in E. coli.
C-Terminus
Detects a band of 37 kDa by Western blot in the following human (293A, HMEC-1, U-118, HaCat), rat (TR-iBRB), mouse (AtT-20, Hepa), canine (D17) and monkey (COS-7) whole cell lysates.

Packaging

PBS, 20% glycerol, 0.05% sodium azide.
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only.

About GAPDH

P04406 NM_002046 NP_002037.2

GAPDH Antibody, A1 40 kd subunit Antibody, Activator 1 40 kd subunit Antibody, G3PD Antibody, GAPD Antibody, G3pdh Antibody, Rfc40 Antibody, Rf-c 40 kd subunit Antibody

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton.

Immunohistochemistry

Immunohistochemistry
Anti-GAPDH antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9773 concentration 5 ug/ml.

Western blot

Western blot
Western blot. Anti-GAPDH antibody at 1:500 dilution. Lysates at 50 ug per lane. Rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution.

Requested From: United States
Date Requested: 12/5/2016

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