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Anti-GAPDH Antibody (C-Terminus, DY488) LS-C143008

Ordering

Wt. Vol. Conc. Price
- 100 µl - Unavailable

Most Popular GAPDH Antibodies

Anti-GAPDH Antibody (C-Terminus) LS-C51798
Rabbit Polyclonal to Human GAPDH
Human, Mouse, Rat, Dog, Pig, Chicken
Western blot
Unconjugated
Anti-GAPDH Antibody IHC-plus™ LS-B4075
Rabbit Polyclonal to Human GAPDH
Human, Mouse, Rat, Drosophila
IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image
Anti-GAPDH Antibody (clone 4G5) IHC-plus™ LS-B8020
Mouse Monoclonal [clone 4G5] (IgG1) to Human GAPDH
Human, Mouse, Rat, Bovine, Cat, Dog, Goat, Pig, Rabbit, Fish
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Mouse GAPDH
Mouse
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
DY488 Conjugated

Details

Mouse GAPDH
Rabbit
Mouse (tested or 100% immunogen sequence identity)
Polyclonal
DyLight 488. Also available Unconjugated or conjugated with Biotin, DY650, DY550.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin
  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

GAPDH antibody was raised against the epitope recognized by this antibody maps to a region between residues 300 and the C-terminus (residue 335) of mouse Glyceraldehyde-3-Phosphate Dehydrogenase using the numbering given in entry NP_001001303.1 (GeneID 407972)
C-Terminus
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

50 mM sodium borate
Store at 4°C. Do not freeze.
For research use only.

About GAPDH

P04406 NM_002046 NP_002037.2

GAPDH Antibody, A1 40 kd subunit Antibody, Activator 1 40 kd subunit Antibody, G3PD Antibody, GAPD Antibody, G3pdh Antibody, Rfc40 Antibody, Rf-c 40 kd subunit Antibody

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton.

Immunohistochemistry

Immunohistochemistry
Anti-GAPDH antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2461 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Detection of Mouse GAPDH by Western Blot. Samples: Whole cell lysate (5, 15 and 50 mcg) from mouse NIH3T3 cells. Antibody: Affinity purified rabbit anti-GAPDH antibody used at 0.04 mcg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 3/29/2017

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