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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GAPDH Antibody (aa92‑103) LS‑C20161
Note: This antibody replaces LS-C66763
GAPDH antibody LS-C20161 is an unconjugated goat polyclonal antibody to GAPDH from human, mouse, rat and other species. Validated for IF, Peptide-ELISA and WB. Cited in 3 publications.
Catalog
Size
Price
LS-C20161-100
100 µg (0.5 mg/ml)
$405
Description
GAPDH antibody LS-C20161 is an unconjugated goat polyclonal antibody to GAPDH from human, mouse, rat and other species. Validated for IF, Peptide-ELISA and WB. Cited in 3 publications.
Target
Human GAPDH
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Dog, Hamster, Pig, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Immunofluorescence
  • Western blot (0.1 - 0.3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:4000)
Immunogen
GAPDH antibody was raised against synthetic peptide C-GVNHEKYDNSLK from an internal region of human GAPDH (NP_002037.2). Percent identity by BLAST analysis: Human, Orangutan, Baboon, Monkey, Mouse, Rat, Ferret, Hamster, Elephant, Dog, Cat, Rabbit, Pig, Opossum, Salmon (100%).
Blocking Peptide
LS-E26015 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C20161. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa92-103
Specificity
Human GAPDH. GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About GAPDH
P04406 NM_002046 NP_002037.2

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 This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Publications (3)

1
Elucidating the phenomenon of HESC-derived RPE: anatomy of cell genesis, expansion and retinal transplantation. Vugler A, Carr AJ, Lawrence J, Chen LL, Burrell K, Wright A, Lundh P, Semo M, Ahmado A, Gias C, da Cruz L, Moore H, Andrews P, Walsh J, Coffey P. Experimental neurology. 2008 214:347-61. [PubMed:18926821]
2
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3
The expression of retinal cell markers in human retinal pigment epithelial cells and their augmentation by the synthetic retinoid fenretinide. Carr AJ, Vugler AA, Yu L, Semo M, Coffey P, Moss SE, Greenwood J. Molecular vision. 2011 17:1701-15. [PubMed:21738400] [PMC:PMC3130725]

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Images

Immunofluorescence

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and plasma membrane staining. The nucle
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and plasma membrane staining. The nucle

Western blot

(0.1 ug/ml) staining of Human Tonsil Lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
(0.1 ug/ml) staining of Human Tonsil Lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunofluorescence

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and plasma membrane staining. The nucle
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and plasma membrane staining. The nucle

Western blot

(0.1 ug/ml) staining of Human Tonsil Lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
(0.1 ug/ml) staining of Human Tonsil Lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunofluorescence

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and plasma membrane staining. The nucle
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and plasma membrane staining. The nucle

Western blot

(0.1 ug/ml) staining of Human Tonsil Lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
(0.1 ug/ml) staining of Human Tonsil Lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunofluorescence

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and plasma membrane staining. The nucle
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and plasma membrane staining. The nucle

Western blot

(0.1 ug/ml) staining of Human Tonsil Lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
(0.1 ug/ml) staining of Human Tonsil Lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: United States
Date Requested: 12/9/2018
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