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Anti-FZD4 / Frizzled 4 Antibody (Extracellular Domain) LS-C6904

Ordering

Wt. Vol. Conc. Price
50 µg 100 µl 0.5 mg/ml $385
100 µg - - $530
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Rat Monoclonal (IgG2a) to Mouse FZD4 / Frizzled 4
Mouse, Human
ICC, Western blot, Flow Cytometry
Unconjugated

Details

Mouse FZD4 / Frizzled 4
Rat
Mouse, Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal
Protein G purified

Applications

ICC (10 µg/ml), Western blot (1 - 2 µg/ml), Flow Cytometry (100 µg/ml)

Specificity and Use

FZD4 / Frizzled 4 antibody was raised against nS0-derived rmFrizzled-4 extracellular domain.
Extracellular Domain
Recognizes mouse Frizzled-4. Shows no cross-reactivity with rmFrizzled-1, -2, 3, -6, -7 -8 or rhFrizzled-5. Species cross-reactivity: Human. Detects Frizzled-4 by Flow Cytometry and Immunohistochemistry on mouse differentiated embryoid bodies derived from the mouse embryonic cell line, D3. Also recognizes human Frizzled-4 by Flow Cytometry and Immunohistochemistry on HEK 293 cells.
Suitable for use in Flow Cytometry, Immunocytochemistry and Western Blot. Flow Cytometry: 100 ug/ml. Add 10 ul to 1-2.5x10^5 cells. Total reaction volume should not exceeding 200ul. Immunocytochemistry: 10 ug/ml in 4% paraformaldehyde-fixed cells. Western Blot:1-2 ug/ml. The detection limit is ~5 ng/lane under non-reducing and reducing conditions.

Packaging

PBS, pH 7.2, 5% trehalose.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

Publications (1)

1
Tumor suppressor microRNA-493 decreases cell motility and migration ability in human bladder cancer cells by downregulating RhoC and FZD4. Ueno K, Hirata H, Majid S, Yamamura S, Shahryari V, Tabatabai ZL, Hinoda Y, Dahiya R. Molecular cancer therapeutics. 2012 Jan;11:244-53. [Full Text Article] [PubMed:22057916] [PMC:PMC3940358]

About FZD4 / Frizzled 4

Q9ULV1 NM_012193 NP_036325.2

FZD4 Antibody, CD344 Antibody, CD344 antigen Antibody, EVR1 Antibody, Frizzled family receptor 4 Antibody, Frizzled-4 Antibody, Fz-4 Antibody, Fz4 Antibody, HFz4 Antibody, Exudative vitreoretinopathy 1 Antibody, FEVR Antibody, FZD4S Antibody, FzE4 Antibody, Frizzled 4 Antibody, Frizzled homolog 4 Antibody, WNT receptor frizzled-4 Antibody

Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin (CTNNB1) canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin (CTNNB1) and activation of Wnt target genes. Plays a critical role in retinal vascularization by acting as a receptor for Wnt proteins and norrin (NDP).

Requested From: United States
Date Requested: 10/1/2016

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