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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FIG4 Antibody (aa135‑430) LS‑C186126
FIG4 antibody LS-C186126 is an unconjugated rabbit polyclonal antibody to human FIG4. Validated for ICC, IHC and WB.
Catalog
Size
Price
LS-C186126-100
100 µl (1 mg/ml)
Unavailable

Popular FIG4 Products

FIG4 Antibody western blot of mouse kidney tissue lysates (35 ug/lane). The FIG4 antibody detected the FIG4 protein (arrow).
Species: Human, Mouse
Applications: Western blot
Antibody
Species: Mouse, Human, Rat
Applications: IHC, ICC, Western blot
Antibody
Species: Mouse, Human, Rat
Applications: IHC, ICC, Western blot
Antibody
Species: Mouse, Human, Rat
Applications: IHC, ICC, Western blot

Product Description

FIG4 antibody LS-C186126 is an unconjugated rabbit polyclonal antibody to human FIG4. Validated for ICC, IHC and WB.
About FIG4
FIG4 belongs to the SAC domain-containing protein gene family. The SAC domain, approximately 400 amino acids in length and consisting of seven conserved motifs, has been shown to possess phosphoinositide phosphatase activity. The yeast homolog, Sac1p, is involved in the regulation of various phosphoinositides, and affects diverse cellular functions such as actin cytoskeleton organization, Golgi function, and maintenance of vacuole morphology. Q92562 NM_014845 NP_055660.1

FIG4 Antibody, ALS11 Antibody, CMT4J Antibody, HSac3 Antibody, KIAA0274 Antibody, RP1-249I4.1 Antibody, DJ249I4.1 Antibody, FIG4 homolog (S. cerevisiae) Antibody, SAC3 Antibody


Specifications

Target
Human FIG4
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey, Mouse, Rat, Bovine, Dog, Pig, Chicken (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:1000)
  • ICC (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
FIG4 antibody was raised against recombinant fragment corresponding to a region within amino acids 135 and 430 of FIG4 (SwissProt Q92562). Percent identity by BLAST analysis: Human (100%); Monkey (99%); Dog (98%); Pig (97%); Bovine (96%); Mouse (95%); Rat (93%); Chicken (90%).
Epitope
aa135-430
Specificity
Human KIAA0274 / SAC3
Usage
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
Presentation
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded Breast ca using FIG4 antibody at 1:500 dilution.
IHC of paraffin-embedded Breast ca using FIG4 antibody at 1:500 dilution.

Immunocytochemistry

FIG4 antibody detects FIG4 protein at cytoplasm by immunofluorescent analysis. HeLa cells were fixed in ice-cold MeOH for 5 min. FIG4 protein stained by FIG4 antibody diluted at 1:500.
FIG4 antibody detects FIG4 protein at cytoplasm by immunofluorescent analysis. HeLa cells were fixed in ice-cold MeOH for 5 min. FIG4 protein stained by FIG4 antibody diluted at 1:500.

Western blot

Sample (30 ug of whole cell lysate). A: HCT116. 7.5% SDS PAGE. KIAA0274 / SAC3 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: HCT116. 7.5% SDS PAGE. KIAA0274 / SAC3 antibody diluted at 1:1000.

Immunohistochemistry

IHC of paraffin-embedded Breast ca using FIG4 antibody at 1:500 dilution.
IHC of paraffin-embedded Breast ca using FIG4 antibody at 1:500 dilution.

Immunocytochemistry

FIG4 antibody detects FIG4 protein at cytoplasm by immunofluorescent analysis. HeLa cells were fixed in ice-cold MeOH for 5 min. FIG4 protein stained by FIG4 antibody diluted at 1:500.
FIG4 antibody detects FIG4 protein at cytoplasm by immunofluorescent analysis. HeLa cells were fixed in ice-cold MeOH for 5 min. FIG4 protein stained by FIG4 antibody diluted at 1:500.

Western blot

Sample (30 ug of whole cell lysate). A: HCT116. 7.5% SDS PAGE. KIAA0274 / SAC3 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: HCT116. 7.5% SDS PAGE. KIAA0274 / SAC3 antibody diluted at 1:1000.

Immunohistochemistry

IHC of paraffin-embedded Breast ca using FIG4 antibody at 1:500 dilution.
IHC of paraffin-embedded Breast ca using FIG4 antibody at 1:500 dilution.

Immunocytochemistry

FIG4 antibody detects FIG4 protein at cytoplasm by immunofluorescent analysis. HeLa cells were fixed in ice-cold MeOH for 5 min. FIG4 protein stained by FIG4 antibody diluted at 1:500.
FIG4 antibody detects FIG4 protein at cytoplasm by immunofluorescent analysis. HeLa cells were fixed in ice-cold MeOH for 5 min. FIG4 protein stained by FIG4 antibody diluted at 1:500.

Western blot

Sample (30 ug of whole cell lysate). A: HCT116. 7.5% SDS PAGE. KIAA0274 / SAC3 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: HCT116. 7.5% SDS PAGE. KIAA0274 / SAC3 antibody diluted at 1:1000.

Immunohistochemistry

IHC of paraffin-embedded Breast ca using FIG4 antibody at 1:500 dilution.
IHC of paraffin-embedded Breast ca using FIG4 antibody at 1:500 dilution.

Immunocytochemistry

FIG4 antibody detects FIG4 protein at cytoplasm by immunofluorescent analysis. HeLa cells were fixed in ice-cold MeOH for 5 min. FIG4 protein stained by FIG4 antibody diluted at 1:500.
FIG4 antibody detects FIG4 protein at cytoplasm by immunofluorescent analysis. HeLa cells were fixed in ice-cold MeOH for 5 min. FIG4 protein stained by FIG4 antibody diluted at 1:500.

Western blot

Sample (30 ug of whole cell lysate). A: HCT116. 7.5% SDS PAGE. KIAA0274 / SAC3 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: HCT116. 7.5% SDS PAGE. KIAA0274 / SAC3 antibody diluted at 1:1000.


Requested From: 
Date Requested: 6/17/2018

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