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Anti-FIG4 Antibody (aa135-430) LS-C186126

Ordering

Wt. Vol. Conc. Price
- 100 µl 1 mg/ml $335
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular FIG4 Antibodies

Anti-FIG4 Antibody (aa846-874) LS-C159657
Rabbit Polyclonal to Human FIG4
Human, Mouse
Western blot
Unconjugated
Western blot Image
Anti-FIG4 Antibody (aa846-874, APC) LS-C257285
Rabbit Polyclonal (IgG) to Human FIG4
Human, Mouse
Western blot, ELISA
APC Conjugated
Anti-FIG4 Antibody (aa846-874, FITC) LS-C257288
Rabbit Polyclonal (IgG) to Human FIG4
Human, Mouse
Western blot, ELISA
FITC Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human FIG4
Human
IHC - Paraffin, ICC, Western blot
Unconjugated

Details

Human FIG4
Rabbit
Human (tested or 100% immunogen sequence identity)
Monkey, Mouse, Rat, Bovine, Dog, Pig, Chicken (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:100 - 1:1000)
  • ICC (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)

Specificity and Use

FIG4 antibody was raised against recombinant fragment corresponding to a region within amino acids 135 and 430 of FIG4 (SwissProt Q92562). Percent identity by BLAST analysis: Human (100%); Monkey (99%); Dog (98%); Pig (97%); Bovine (96%); Mouse (95%); Rat (93%); Chicken (90%).
aa135-430
Human KIAA0274 / SAC3
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).

Packaging

0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
For research use only.

About FIG4

Q92562 NM_014845 NP_055660.1

FIG4 Antibody, ALS11 Antibody, CMT4J Antibody, HSac3 Antibody, KIAA0274 Antibody, RP1-249I4.1 Antibody, DJ249I4.1 Antibody, FIG4 homolog (S. cerevisiae) Antibody, SAC3 Antibody

FIG4 belongs to the SAC domain-containing protein gene family. The SAC domain, approximately 400 amino acids in length and consisting of seven conserved motifs, has been shown to possess phosphoinositide phosphatase activity. The yeast homolog, Sac1p, is involved in the regulation of various phosphoinositides, and affects diverse cellular functions such as actin cytoskeleton organization, Golgi function, and maintenance of vacuole morphology.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Breast ca using FIG4 antibody at 1:500 dilution.

Immunocytochemistry

Immunocytochemistry
FIG4 antibody detects FIG4 protein at cytoplasm by immunofluorescent analysis. HeLa cells were fixed in ice-cold MeOH for 5 min. FIG4 protein stained by FIG4 antibody diluted at 1:500.

Western blot

Western blot
Sample (30 ug of whole cell lysate). A: HCT116. 7.5% SDS PAGE. KIAA0274 / SAC3 antibody diluted at 1:1000.
 

Requested From: United States
Date Requested: 12/4/2016

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