Target
Human FBX09 / FBXO9
Synonyms
FBXO9 | F-box protein Fbx9 | FBX09 | FBX9 | NY-REN-57 | VCIA1 | DJ341E18.2 | F-box only protein 9 | F-box protein 9
Reactivity
Human
(tested or 100% immunogen sequence identity)
Predicted
Human, Chimpanzee, Mouse, Rat, Bovine, Dog, Zebrafish (at least 90% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 425-447 of human FBOX9 protein.
Specificity
A BLAST analysis was used to suggest cross reactivity with FBOX9 protein from human, dog, rat and chimpanzee based on 100% homology with the immunizing sequence. Expect partial reactivity with homologues from zebrafish, bovine and mouse (94% homology) as well as chicken (88%) and Xenopus laevis (82%). Reactivity is expected against all known isoforms of this protein, as the immunogen sequence is common to their carboxy-terminal ends. Reactivity against homologues from other sources is not known.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (1:1000)
- ELISA (1:5000 - 1:25000)
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Usage
Immunohistochemistry: LS-B371 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B371 was determined to be 5 ug/ml.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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