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Anti-FBL / FIB / Fibrillarin Antibody (clone 28F2) LS-C204565

Catalog Size Price
LS-C204565-500 500 µl Unavailable

Most Popular FBL / FIB / Fibrillarin Antibodies

Anti-FBL / FIB / Fibrillarin Antibody (clone 38F3) LS-C2552
Mouse Monoclonal [clone 38F3] (IgG1) to Rat FBL / FIB / Fibrillarin
Rat, Human, Mouse, Chicken, Drosophila, Yeast, C. elegans, Plant
IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunofluorescence Image
Anti-FBL / FIB / Fibrillarin Antibody (aa61-110) LS-C30767
Rabbit Polyclonal (IgG) to Human FBL / FIB / Fibrillarin
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse
IHC - Paraffin, Western blot
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Immunohistochemistry Image
Anti-FBL / FIB / Fibrillarin Antibody (clone 38F3) LS-C204517
Mouse Monoclonal [clone 38F3] (IgG1) to Yeast FBL / FIB / Fibrillarin
Yeast, Human, Rat
ICC, Immunofluorescence, Western blot
Unconjugated
Immunocytochemistry Image
Anti-FBL / FIB / Fibrillarin Antibody LS-C335127
Rabbit Polyclonal (IgG) to Human FBL / FIB / Fibrillarin
Human, Mouse, Rat
IHC, Immunofluorescence, Western blot, Immunoprecipitation, RNA Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-FBL / FIB / Fibrillarin Antibody (aa271-321) IHC-plus™ LS-B13538
Rabbit Polyclonal (IgG) to Human FBL / FIB / Fibrillarin
Human, Mouse, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed
Mouse Monoclonal [clone 28F2] (IgG1) to Yeast FBL / FIB / Fibrillarin
Yeast
Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated

Details

Yeast FBL / FIB / Fibrillarin
Mouse
Yeast (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [28F2]
Unconjugated
Tissue culture supernatant
Unmodified

Applications

  • Immunofluorescence (1:5000)
  • Western blot (1:10000 - 1:25000)
  • Immunoprecipitation

Specificity and Use

To raise this antibody, mice were injected with yeast nuclear preparations and hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates.
This monoclonal antibody does not cross-react with fibrillarin in other species, but is an excellent marker of the nucleolus of yeast cells.
For western blots of yeast protein samples, use FBL / FIB / Fibrillarin antibody diluted 1:10000 (cell lysates) to 1:25000 (nuclear fractions), followed by chemiluminescent detection (ECL). For other (non-ECL) western detection methods, try the antibody diluted 1:1000-1:5000. For immunofluorescence on yeast cells, use the antibody diluted 1:2000-1:20000. We routinely dilute to 1:5000. For immunoprecipitations, use 2-4 ul for an amount of lysate derived from 5 OD600 units of yeast culture.

Packaging

Tissue culture supernatant, 10 mM sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About FBL / FIB / Fibrillarin

P22087 NM_001436 NP_001427.2

FBL Antibody, FLRN Antibody, FBRL Antibody, FIB1 Antibody, Fibrillarin Antibody, RNU3IP1 Antibody, FIB Antibody

S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA.

Western blot

Western blot
Western blots of whole yeast protein extracts with a collection of our antibodies. The blot for FBL / FIB / Fibrillarin antibody is in the indicated lane, and the number indicates the SDS-PAGE molecular weight in kiloDaltons.

Requested From: 
Date Requested: 10/20/2017

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