Yeast FBL / FIB / Fibrillarin

Mouse

Yeast (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

28F2

Unconjugated

Tissue culture supernatant

Unmodified

To raise this antibody, mice were injected with yeast nuclear preparations and hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates.

This monoclonal antibody does not cross-react with fibrillarin in other species, but is an excellent marker of the nucleolus of yeast cells.

• Immunofluorescence (1:5000)
• Western blot (1:10000 - 1:25000)
• Immunoprecipitation

For western blots of yeast protein samples, use FBL / FIB / Fibrillarin antibody diluted 1:10000 (cell lysates) to 1:25000 (nuclear fractions), followed by chemiluminescent detection (ECL). For other (non-ECL) western detection methods, try the antibody diluted 1:1000-1:5000. For immunofluorescence on yeast cells, use the antibody diluted 1:2000-1:20000. We routinely dilute to 1:5000. For immunoprecipitations, use 2-4 ul for an amount of lysate derived from 5 OD600 units of yeast culture.

Tissue Culture Supernatant, 10 mM Sodium Azide

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P22087 NCBI: NM_001436 NP_001427.2