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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C204517-500 500 µl $285 
FBL / FIB / Fibrillarin Antibody - Human SH-SY5Y cells stained with FBL / FIB / Fibrillarin antibody, showing prominent specular nucleolar staining. The nuclei are counter stained with blue DAPI DNA stain, so these spots appear very pale blue. The cells are also stained with chicken antibody to neurofilament NF-H.
FBL / FIB / Fibrillarin Antibody - Strip blots of yeast protein extracts stained with the indicated antibodies. TargetName] antibody is first lane on the left and stains a single band at ~34kDa.
FBL / FIB / Fibrillarin Antibody - Human SH-SY5Y cells stained with FBL / FIB / Fibrillarin antibody, showing prominent specular nucleolar staining. The nuclei are counter stained with blue DAPI DNA stain, so these spots appear very pale blue. The cells are also stained with chicken antibody to neurofilament NF-H.
FBL / FIB / Fibrillarin Antibody - Strip blots of yeast protein extracts stained with the indicated antibodies. TargetName] antibody is first lane on the left and stains a single band at ~34kDa.
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Monoclonal FBL / FIB / Fibrillarin Antibody (clone 38F3, IF, WB) LS‑C204517

Monoclonal FBL / FIB / Fibrillarin Antibody (clone 38F3, IF, WB) LS‑C204517

Note: This antibody replaces LS-C66045, LS-C2552, LS-C143055
Antibody:
Mouse Monoclonal to Yeast FBL / FIB / Fibrillarin
Application:
ICC, IF, WB
Reactivity:
Yeast, Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$285 
LS-C204517-500
500 µl
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Yeast FBL / FIB / Fibrillarin
Application:
ICC, IF, WB
Reactivity:
Yeast, Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Fibrillarin antibody LS-C204517 is an unconjugated mouse monoclonal antibody to Fibrillarin (FBL / FIB ) from human and rat. Validated for ICC, IF and WB.
Target
Yeast FBL / FIB / Fibrillarin
Synonyms
FBL | FLRN | FBRL | FIB1 | Fibrillarin | RNU3IP1 | FIB
Host
Mouse
Reactivity
Yeast, Human, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
38F3
Conjugations
Unconjugated
Purification
Tissue culture supernatant
Modifications
Unmodified
Applications
  • ICC (1:1000 - 1:5000)
  • Immunofluorescence (1:1000 - 1:5000)
  • Western blot (1:2000 - 1:10000)
Immunogen
Mice were injected with yeast nuclear preparations and hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates.
Specificity
This clone stains a single ~34kDa band on western blotting and shows a clear and strong punctate staining of yeast nuclei. Subsequently it was found that this antibody recognizes fibrillarin in a wide variety of species including human, rat, Drosophila, S. pombe, C. elegans, and plants. It can therefore be used to identify nucleoli immunocytochemically.
Usage
For western blots of yeast protein samples, use FBL / FIB / Fibrillarin antibody diluted 1:2000 (cell lysates) to 1:10000 (nuclear fractions), followed by chemiluminescent detection (ECL). For other (non-ECL) western detection methods, try the antibody diluted 1:1000-1:5000. To detect mammalian fibrillarin on western blots by ECL, try the antibody at 1:500 dilution. For immunofluorescence on yeast cells, use the antibody diluted 1:1000-1:5000. For IF of mammalian cells, try the antibody at 1:500.
Presentation
Tissue Culture Supernatant, 10 mM Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FBL / FIB / Fibrillarin
P22087 NM_001436 NP_001427.2

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Requested From: United States
Date Requested: 2/22/2020