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Anti-FASN / Fatty Acid Synthase Antibody (aa942-973) LS-C159624

Ordering

Wt. Vol. Conc. Price
- 100 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular FASN / Fatty Acid Synthase Antibodies

Anti-FASN / Fatty Acid Synthase Antibody (aa942-973) IHC-plus™ LS-B3636
Rabbit Polyclonal to Human FASN / Fatty Acid Synthase
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgM) to Human FASN / Fatty Acid Synthase
Human, Mouse
Immunofluorescence, Western blot
Unconjugated

Details

Human FASN / Fatty Acid Synthase
Mouse
Human, Mouse (tested or 100% immunogen sequence identity)
IgM Monoclonal
Unconjugated
Ascites
Unmodified

Applications

  • Immunofluorescence (1:200)
  • Western blot (1:100 - 1:1000)

Specificity and Use

FASN / Fatty Acid Synthase antibody was raised against kLH-conjugated synthetic peptide between amino acids 942-973 from internal region of human FASN.
aa942-973
Human FASN / Fatty Acid Synthase

Packaging

Ascites, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About FASN / Fatty Acid Synthase

P49327 NM_004104 NP_004095.4

FASN Antibody, Fatty acid synthase Antibody, OA-519 Antibody, SDR27X1 Antibody

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

Immunofluorescence

Immunofluorescence
Fluorescent confocal image of HepG2 cells stained with FASN antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated FASN primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the FASN immunosignal to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000169710).

Western blot

Western blot
FASN Antibody western blot of mouse brain tissue lysates (35 ug/lane). The FASN antibody detected the FASN protein (arrow).

Requested From: United States
Date Requested: 12/9/2016

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