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Catalog Number Size Price
LS-C408077-10 10 µg $318 
LS-C408077-100 100 µg $470 
FASN / Fatty Acid Synthase Antibody - IHC analysis of FASN using anti-FASN antibody. FASN was detected in immunocytochemical section of MCF-7 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-FASN Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
FASN / Fatty Acid Synthase Antibody - IHC analysis of FASN using anti-FASN antibody. FASN was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-FASN Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
FASN / Fatty Acid Synthase Antibody - FASN was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti- FASN Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.
FASN / Fatty Acid Synthase Antibody - Western blot analysis of FASN expression in HEPG2 whole cell lysates (lane 1), U87 whole cell lysates (lane 2) and RAJI whole cell lysates (lane 3). FASN at 273KD was detected using rabbit anti-FASN Antigen Affinity purified polyclonal antibody at0.5 ug/ml. The blot was developed using chemiluminescence (ECL) method.
FASN / Fatty Acid Synthase Antibody - Western blot analysis of FASN expression in HEPG2 whole cell lysates (lane 1), U87 whole cell lysates (lane 2) and RAJI whole cell lysates (lane 3). FASN at 273 kD was detected using rabbit anti- FASN Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.
FASN / Fatty Acid Synthase Antibody - IHC analysis of FASN using anti-FASN antibody. FASN was detected in immunocytochemical section of MCF-7 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-FASN Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
FASN / Fatty Acid Synthase Antibody - IHC analysis of FASN using anti-FASN antibody. FASN was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-FASN Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
FASN / Fatty Acid Synthase Antibody - FASN was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti- FASN Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.
FASN / Fatty Acid Synthase Antibody - Western blot analysis of FASN expression in HEPG2 whole cell lysates (lane 1), U87 whole cell lysates (lane 2) and RAJI whole cell lysates (lane 3). FASN at 273KD was detected using rabbit anti-FASN Antigen Affinity purified polyclonal antibody at0.5 ug/ml. The blot was developed using chemiluminescence (ECL) method.
FASN / Fatty Acid Synthase Antibody - Western blot analysis of FASN expression in HEPG2 whole cell lysates (lane 1), U87 whole cell lysates (lane 2) and RAJI whole cell lysates (lane 3). FASN at 273 kD was detected using rabbit anti- FASN Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.
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Polyclonal Rabbit anti‑Human FASN / Fatty Acid Synthase Antibody (aa1‑226, IHC, WB) LS‑C408077

Polyclonal Rabbit anti‑Human FASN / Fatty Acid Synthase Antibody (aa1‑226, IHC, WB) LS‑C408077

Antibody:
FASN / Fatty Acid Synthase Rabbit anti-Human Polyclonal (aa1-226) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C408077-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
FASN / Fatty Acid Synthase Rabbit anti-Human Polyclonal (aa1-226) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Fatty Acid Synthase antibody LS-C408077 is an unconjugated rabbit polyclonal antibody to human Fatty Acid Synthase (FASN) (aa1-226). Validated for IHC and WB.
Target
Human FASN / Fatty Acid Synthase
Synonyms
FASN | Fatty acid synthase | OA-519 | SDR27X1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived human FASN recombinant protein (Position: M1-E226). Human FASN shares 90.3% and 90.7% amino acid (aa) sequence identity with mouse and rat FASN, respectively.
Epitope
aa1-226
Specificity
Ubiquitous. Prominent expression in brain, lung, and liver. .
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FASN / Fatty Acid Synthase
P49327 NM_004104 NP_004095.4

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/2/2024