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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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F10 / Factor X Antibody (aa33‑312) LS‑C186101
Factor X antibody LS-C186101 is an unconjugated rabbit polyclonal antibody to human Factor X (F10). Validated for ICC, IHC and WB.
Catalog
Size
Price
LS-C186101-100
100 µl (1 mg/ml)
Unavailable

Popular F10 / Factor X Products

Antibody
Species: Human
Applications: ELISA, Immunoelectrophoresis
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Dog, Guinea pig, Pig
Applications: Western blot
Antibody
Species: Mouse
Applications: ELISA
Anti-F10 / Factor X antibody IHC of human liver, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4235 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, ELISA, Immunodiffusion, Immunoelectrophoresis
Western blot of extracts from A549 cells, treated with etoposide 24 uM 24h, using FA10 (light chain, Cleaved-Ala41) Antibody. The lane on the right is treated with the synthesized peptide.
Species: Human
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

Factor X antibody LS-C186101 is an unconjugated rabbit polyclonal antibody to human Factor X (F10). Validated for ICC, IHC and WB.
About F10 / Factor X
F10 / Factor X is the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. P00742 NM_000504 NP_000495.1

F10 Antibody, Factor X Antibody, FX Antibody, Factor X deficiency Antibody, Stuart factor Antibody, Stuart-Prower factor Antibody, Coagulation factor X Antibody, Factor Xa Antibody, FXA Antibody, Prothrombinase Antibody


Specifications

Target
Human F10 / Factor X
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:1000)
  • ICC (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
F10 / Factor X antibody was raised against recombinant fragment corresponding to a region within amino acids 33 and 312 of Factor X (SwissProt P00742).
Epitope
aa33-312
Specificity
Human Factor X
Usage
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
Presentation
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded A549 xenograft using Factor X antibody at 1:500 dilution.
IHC of paraffin-embedded A549 xenograft using Factor X antibody at 1:500 dilution.

Immunocytochemistry

Immunofluorescence of methanol-fixed HeLa using Factor X antibody at 1:200 dilution.
Immunofluorescence of methanol-fixed HeLa using Factor X antibody at 1:200 dilution.

Western blot

Factor X antibody detects F10 protein by Western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug whole cell lysate/extract of human F10-transfected 293T cells. 10 % SDS-PAGE. Factor X antibody dilution:1:5000
Factor X antibody detects F10 protein by Western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug whole cell lysate/extract of human F10-transfected 293T cells. 10 % SDS-PAGE. Factor X antibody dilution:1:5000

Immunohistochemistry

IHC of paraffin-embedded A549 xenograft using Factor X antibody at 1:500 dilution.
IHC of paraffin-embedded A549 xenograft using Factor X antibody at 1:500 dilution.

Immunocytochemistry

Immunofluorescence of methanol-fixed HeLa using Factor X antibody at 1:200 dilution.
Immunofluorescence of methanol-fixed HeLa using Factor X antibody at 1:200 dilution.

Western blot

Factor X antibody detects F10 protein by Western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug whole cell lysate/extract of human F10-transfected 293T cells. 10 % SDS-PAGE. Factor X antibody dilution:1:5000
Factor X antibody detects F10 protein by Western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug whole cell lysate/extract of human F10-transfected 293T cells. 10 % SDS-PAGE. Factor X antibody dilution:1:5000

Immunohistochemistry

IHC of paraffin-embedded A549 xenograft using Factor X antibody at 1:500 dilution.
IHC of paraffin-embedded A549 xenograft using Factor X antibody at 1:500 dilution.

Immunocytochemistry

Immunofluorescence of methanol-fixed HeLa using Factor X antibody at 1:200 dilution.
Immunofluorescence of methanol-fixed HeLa using Factor X antibody at 1:200 dilution.

Western blot

Factor X antibody detects F10 protein by Western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug whole cell lysate/extract of human F10-transfected 293T cells. 10 % SDS-PAGE. Factor X antibody dilution:1:5000
Factor X antibody detects F10 protein by Western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug whole cell lysate/extract of human F10-transfected 293T cells. 10 % SDS-PAGE. Factor X antibody dilution:1:5000

Immunohistochemistry

IHC of paraffin-embedded A549 xenograft using Factor X antibody at 1:500 dilution.
IHC of paraffin-embedded A549 xenograft using Factor X antibody at 1:500 dilution.

Immunocytochemistry

Immunofluorescence of methanol-fixed HeLa using Factor X antibody at 1:200 dilution.
Immunofluorescence of methanol-fixed HeLa using Factor X antibody at 1:200 dilution.

Western blot

Factor X antibody detects F10 protein by Western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug whole cell lysate/extract of human F10-transfected 293T cells. 10 % SDS-PAGE. Factor X antibody dilution:1:5000
Factor X antibody detects F10 protein by Western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug whole cell lysate/extract of human F10-transfected 293T cells. 10 % SDS-PAGE. Factor X antibody dilution:1:5000


Requested From: 
Date Requested: 6/25/2018

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