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Anti-EWSR1 / EWS Antibody (clone 5H7) LS-C204515

Note: This antibody replaces LS-C180201

Ordering

Wt. Vol. Conc. Price
- 100 µl - $285
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular EWSR1 / EWS Antibodies

Anti-EWSR1 / EWS Antibody (aa201-215) IHC-plus™ LS-B3967
Goat Polyclonal to Human EWSR1 / EWS
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Pig
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-EWSR1 / EWS Antibody (clone 5C10) IHC-plus™ LS-B6082
Mouse Monoclonal [clone 5C10] (IgG2a,k) to Human EWSR1 / EWS
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-EWSR1 / EWS Antibody LS-C346054
Rabbit Polyclonal (IgG) to Human EWSR1 / EWS
Human, Mouse
IHC, Immunofluorescence, Western blot
Unconjugated
Immunofluorescence Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 5H7] (IgG2b,k) to Human EWSR1 / EWS
Human
ICC, Immunofluorescence, Western blot
Unconjugated

Details

Human EWSR1 / EWS
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2b,k Monoclonal [5H7]
Unconjugated
Protein G purified
Unmodified

Applications

  • ICC (1:500)
  • Immunofluorescence (1:500)
  • Western blot (1:1000)

Specificity and Use

EWSR1 / EWS antibody was raised against full length recombinant human EWS expressed in and purified from E. coli.
Clone 5H7 recognizes EWS specifically both in western blots and in immunocytochemical experiments. On blots clone 5H7 reveals a prominent 85 kDa band, and on cells in tissue culture the antibody stains mainly the nucleus.
The antibody solution can be used at dilutions of 1:500 for immunofluorescence.&nbsp. For western blots try at 1:1000. A suitable control tissue is HeLa cell lysates.

Packaging

PBS, 0.05% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About EWSR1 / EWS

Q01844 NM_005243 NP_005234.1

EWSR1 Antibody, EWS Antibody, RNA-binding protein EWS Antibody, BK984G1.4 Antibody, EWS oncogene Antibody

EWSR1 / EWS is a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis.

Immunocytochemistry

Immunocytochemistry
An image of HeLa cell cultures stained with antibody EWSR1 / EWS antibody (green) and chicken antibody to vimentin (red). Blue is a DNA stain, and it is clear that in these cells, EWS is localized along with the DNA in the nucleus.

Western blot

Western blot
Blots of whole HeLa cell lysates blotted with EWSR1 / EWS antibody. EWSR1 / EWS antibody binds strongly and cleanly to a band at about 85 kDa. Position at which SDS-PAGE molecular weight standards run is are indicated at left.

Requested From: United States
Date Requested: 12/8/2016

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