Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Estrogen-related receptor alpha (ERR alpha), a NR3 Steroid Receptor, was isolated based on sequence similarity in its DNA-binding domain to estrogen receptor alpha (ER alpha). ERR alpha has been shown to regulate the promoters of lactoferrin, medium-chain acyl CoA dehydrogenase, osteopontin, and thyroid receptor alpha, and it may affect cellular energy balance and bone formation.
Formalin-fixed and paraffin-embedded human kidney carcinoma reacted with ESRRA Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of ESRRA (arrow) using rabbit polyclonal ESRRA Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ESRRA gene.
Western blot of ESRRA Antibody in A375 cell line lysates (35 ug/lane). ESRRA (arrow) was detected using the purified antibody.
Flow cytometric of 293 cells using ESRRA Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.