Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 1D5 + 6F11] to Human ER Alpha / Estrogen Receptor
IHC - Paraffin, Western blot
Mouse Monoclonal [clone 1D5] (IgG1,k) to Human ER Alpha / Estrogen Receptor
Human ER Alpha / Estrogen Receptor
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [1D5]
Western blot (1:50 - 1:100)
Specificity and Use
Recombinant human estrogen receptor protein.
Is specific to estrogen receptor, which is associated with superior prognosis and a better response to endocrine therapy. The nuclei of the estrogen receptor positive cells stain very strongly with this antibody, without any staining in the cytoplasm. However, on cryostat sections a positive staining of estrogen receptor in the nucleus as well as cytoplasm can be seen.
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling.