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Anti-EME1 Antibody (C-Terminus) LS-C111455

Catalog	Size	Price
LS-C111455-50	50 µg	Unavailable

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Reactivity:	Human
Application:	Western blot
Format:	Unconjugated

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Antibody:	Rabbit Polyclonal (IgG) to Human EME1
Reactivity:	Human
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Anti-EME1 Antibody (aa520-561) LS-C408017

Antibody:	Rabbit Polyclonal (IgG) to Human EME1
Reactivity:	Human, Rat
Application:	IHC - Paraffin, Western blot
Format:	Unconjugated

Anti-EME1 Antibody LS-C490477

Antibody:	Rabbit Polyclonal (IgG) to Human EME1
Reactivity:	Human, Rat
Application:	IHC - Paraffin, Western blot
Format:	Unconjugated

Antibody:	Rabbit Polyclonal to Human EME1
Reactivity:	Human, Monkey, Mouse, Bat, Dog, Guinea pig, Pig, Rabbit
Application:	Western blot (applications tested for the base form of this product only)
Format:	Unconjugated

Details

Target:	Human EME1
Host:	Rabbit
Reactivity:	Human, Monkey, Mouse, Bat, Dog, Guinea pig, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted:	Rat, Horse (at least 90% immunogen sequence identity)
Clonality:	Polyclonal
Conjugations:	Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification:	Immunoaffinity purified
Modifications:	Unmodified

Applications

Approved:

Western blot (0.2 - 1 µg/ml)
(applications tested for the base form of this product only)

Specificity and Use

Immunogen:

EME1 antibody was raised against synthetic peptide from C-Terminus of human EME1 (Q96AY2, NP_689676). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Panda, Dog, Bat, Rabbit, Pig (100%); Rat, Elephant, Horse, Platypus (92%); Xenopus (85%).

Blocking Peptide:

LS-E19107 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C111455. Useful for pre-absorption and neutralization of the antibody's antigen binding site.

Epitope:

C-Terminus

Specificity:

Human Eme1

Usage:

Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Presentation:	Lyophilized from PBS with 2% sucrose
Reconstitution:	Distilled water
Storage:	Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
Restrictions:	For research use only.

About EME1

Uniprot: Q96AY2 NCBI: NP_001159603.1

Synonyms: EME1 Antibody, MMS4 Antibody, MMS4L Antibody, MMS4 homolog Antibody, SLX2A Antibody, HMMS4 Antibody

Description: Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.

(More About EME1)

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Western blot

EME1 antibody LS-C111455 Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From:
Date Requested: 6/23/2017

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