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EME1 Antibody (C‑Terminus) LS‑C111455
EME1 antibody LS-C111455 is an unconjugated rabbit polyclonal antibody to EME1 from human, mouse, bat and other species. Validated for WB.
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Species:
Human, Rat
Applications:
IHC, IHC - Paraffin, Western blot
Product Description
EME1 antibody LS-C111455 is an unconjugated rabbit polyclonal antibody to EME1 from human, mouse, bat and other species. Validated for WB.
About EME1
Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.
Q96AY2
NP_001159603.1
EME1 Antibody, MMS4 Antibody, MMS4L Antibody, MMS4 homolog Antibody, SLX2A Antibody, HMMS4 Antibody
Specifications
Target
Human EME1
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Bat, Dog, Guinea pig, Pig, Rabbit
(tested or 100% immunogen sequence identity)
Predicted
Rat, Horse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
- Western blot (0.2 - 1 µg/ml)
- (applications tested for the base form of this product only)
Immunogen
EME1 antibody was raised against synthetic peptide from C-Terminus of human EME1 (Q96AY2, NP_689676). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Panda, Dog, Bat, Rabbit, Pig (100%); Rat, Elephant, Horse, Platypus (92%); Xenopus (85%).
Blocking Peptide
LS-E19107 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C111455. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Immunizing peptide used to generate LS-C111455. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human Eme1
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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Images
Western blot
EME1 antibody LS-C111455 Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
EME1 antibody LS-C111455 Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
EME1 antibody LS-C111455 Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
EME1 antibody LS-C111455 Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Requested From:
Date Requested: 6/21/2018
Date Requested: 6/21/2018
