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Anti-EME1 Antibody (C-Terminus) LS-C111455

Catalog Size Price
LS-C111455-50 50 µg Unavailable

Related Products

18 EME1 Antibodies

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Anti-EME1 Antibody (aa250-330) LS-C383326
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Western blot Image
Anti-EME1 Antibody (aa520-561) LS-C408017
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Anti-EME1 Antibody LS-C490477
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100% Guaranteed
Rabbit Polyclonal to Human EME1
Human, Monkey, Mouse, Bat, Dog, Guinea pig, Pig, Rabbit
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human EME1
Rabbit
Human, Monkey, Mouse, Bat, Dog, Guinea pig, Pig, Rabbit (tested or 100% immunogen sequence identity)
Rat, Horse (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

EME1 antibody was raised against synthetic peptide from C-Terminus of human EME1 (Q96AY2, NP_689676). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Panda, Dog, Bat, Rabbit, Pig (100%); Rat, Elephant, Horse, Platypus (92%); Xenopus (85%).
LS-E19107 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C111455. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
C-Terminus
Human Eme1
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About EME1

Q96AY2 NP_001159603.1

EME1 Antibody, MMS4 Antibody, MMS4L Antibody, MMS4 homolog Antibody, SLX2A Antibody, HMMS4 Antibody

Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.

Western blot

Western blot
EME1 antibody LS-C111455 Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 10/17/2017

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