Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone GP1.4] (IgG1) to Human EMA / MUC1
IHC - Paraffin
Mouse Monoclonal (IgG1) to Human EMA / MUC1
IHC - Paraffin, IHC - Frozen, Western blot
Human EMA / MUC1
Human (tested or 100% immunogen sequence identity)
IHC - Paraffin
IHC - Frozen
Specificity and Use
EMA / MUC1 antibody was raised against human milk fat globulin membrane preparation.
Reactive with 90% of breast carcinoma, most epithelial ovarian carcinoma and a high portion of the lung, urogenital and gastrointestinal carcinomas. Some reactivity is observed in the apical membrane of normal breast epithelium. Weak reactivity is also present in pancreas, kidney and lung. Reacts with a five amino acid epitope of the MUC-1 core protein which is less effective for glycosylation than most other MUC-1 epitopes. Recommended for Mamma carcinoma. Species cross-reactivity: Human.
Suitable for use in Western Blot and Immunohistochemistry (frozen tissue and paraffin sections). Immunohistochemistry: 1:10; Incubation Time 1 hr at room temperature. Antigen Localization: Cytoplasm and membrane.
PBS, pH 7.4, 1% BSA, 0.1% sodium azide.
Short term: +4°C; Long term: Add glycerol (40-50%) -20°C.
EMA / MUC1 is a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas.