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ELK3 / NET Antibody (aa180‑229) LS‑C102854

Catalog Number Size Price
LS-C102854-100 100 µl (0.5 mg/ml) $365 
ELK3 / NET antibody Western blot of Fetal Liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

ELK3 / NET Antibody (aa180‑229) LS‑C102854

Antibody:
Rabbit Polyclonal to Human ELK3 / NET
Application:
WB
Reactivity:
Human, Monkey, Dog, Guinea pig, Horse
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$365 
LS-C102854-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human ELK3 / NET
Application:
WB
Reactivity:
Human, Monkey, Dog, Guinea pig, Horse
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
NET antibody LS-C102854 is an unconjugated rabbit polyclonal antibody to NET (ELK3 ) from human, dog, guinea pig and other species. Validated for WB.
Target
Human ELK3 / NET
Host
Rabbit
Reactivity
Human, Monkey, Dog, Guinea pig, Horse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
Synthetic peptide located between aa180-229 of human ELK3 (P41970, NP_005221). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset (100%); Galago, Mouse, Rat, Hamster, Elephant, Bovine, Rabbit, Zebra finch (92%); Bat (85%); Dog (84%).
Blocking Peptide
LS-E10881 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C102854. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa180-229
Specificity
Human ELK3 / NET
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:12500.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ELK3 / NET
P41970 NM_005230 NP_005221.2

Publications (0)

Reviews (0)

Featured Products

Immunofluorescent analysis of NET (pS357) staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Species: Human, Mouse, Rat, Bovine, Zebrafish
Applications: ICC, Immunofluorescence, Western blot, Immunoprecipitation
Product
Species: Human, Mouse
Applications: Immunofluorescence, ELISA
Reactivity: Mouse
Range: 0.625-40 ng/ml
Anti-Factor I antibody IHC of human colon, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry, ELISA

Requested From: United States
Date Requested: 9/16/2019