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Anti-EIF4EBP1 / 4EBP1 Antibody (phospho-Thr46) LS-C140554

Ordering

Wt. Vol. Conc. Price
- 10 blot - $650
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular EIF4EBP1 / 4EBP1 Antibodies

Anti-EIF4EBP1 / 4EBP1 Antibody (phospho-Thr37/Thr46) LS-C92051
Rabbit Monoclonal (IgG) to Mouse EIF4EBP1 / 4EBP1
Mouse, Human, Monkey, Rat, Drosophila
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Anti-EIF4EBP1 / 4EBP1 Antibody (aa101-118) IHC-plus™ LS-B3748
Rabbit Polyclonal to Human EIF4EBP1 / 4EBP1
Human, Mouse, Rat, Hamster, Rabbit
IHC - Paraffin, Western blot, Gel shift
Unconjugated
Immunohistochemistry Image
Anti-EIF4EBP1 / 4EBP1 Antibody (phospho-Ser65) LS-C122561
Rabbit Polyclonal (IgG) to Human EIF4EBP1 / 4EBP1
Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig, Rabbit
IHC, Western blot, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human EIF4EBP1 / 4EBP1
Human, Mouse
Western blot
Unconjugated

Details

Human EIF4EBP1 / 4EBP1
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

Western blot

Specificity and Use

EIF4EBP1 / 4EBP1 antibody was raised against chemically synthesized phosphopeptide derived from the region of human 4E-BP1 that contains threonine 46. The sequence is conserved in human, mouse and rat.
pThr46
Human EIF4EBP1 / 4EBP1
Optimal concentration should be evaluated by serial dilutions. For Western blotting applications, we recommend using the antibody at a 1:1000 dilution.

Packaging

PBS, pH 7.3, 1.0 mg/ml BSA, 0.05% sodium azide
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
For research use only.

About EIF4EBP1 / 4EBP1

Q13541 NM_004095 NP_004086.1

EIF4EBP1 Antibody, 4E-BP1 Antibody, 4EBP1 Antibody, BP-1 Antibody, PHAS-I Antibody, EIF4E-binding protein 1 Antibody

EIF4EBP1 / 4EBP1 is one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation.

Western blot

Western blot
Lysates prepared from NIH3T3 cells left untreated or treated with PI-3K inhibitor LY2904002 (lanes 2 & 3), prior to PDGF stimulation (lanes 1 & 3) and from EGF-treated HEK293 cells (4-8), Membranes were then left untreated (lanes 1-7) or treated with Lambda phosphatase (8), and incubated with 4E-BP1 [pT46] antibody for two hours at room temperature, following prior incubation with: no peptide (lanes 1 & 2), non-phosphopeptide corresponding to the immunogen (3), a generic phospho-threonine-containing peptide (4), or the phosphopeptide immunogen (5).
 

Requested From: United States
Date Requested: 12/6/2016

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