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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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EIF4EBP1 / 4EBP1 Antibody (aa101‑118) IHC‑plus™ LS‑B3748
Note: This antibody replaces LS-C63173
4EBP1 antibody LS-B3748 is an unconjugated rabbit polyclonal antibody to 4EBP1 (EIF4EBP1) from human, mouse, rat and other species. Validated for GS, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B3748-50
50 µg (0.9 mg/ml)
Unavailable

Popular EIF4EBP1 / 4EBP1 Products

Antibody
Species: Mouse, Human, Rat
Applications: Western blot, Immunoprecipitation, ELISA
IHC of paraffin-embedded human pancreas carcinoma showing cytoplasmic and membrane location using 4E-BP1 antibody with DAB staining.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Antibody
Species: Mouse, Human, Monkey, Rat, Drosophila
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Antibody
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig, Rabbit
Applications: IHC, Western blot, ELISA

Product Description

4EBP1 antibody LS-B3748 is an unconjugated rabbit polyclonal antibody to 4EBP1 (EIF4EBP1) from human, mouse, rat and other species. Validated for GS, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About EIF4EBP1 / 4EBP1
EIF4EBP1 / 4EBP1 is one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. Q13541 NM_004095 NP_004086.1

EIF4EBP1 Antibody, 4E-BP1 Antibody, 4EBP1 Antibody, BP-1 Antibody, PHAS-I Antibody, EIF4E-binding protein 1 Antibody


Specifications

Target
Human EIF4EBP1 / 4EBP1
Host
Rabbit
Reactivity
Human, Mouse, Rat, Hamster, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Monkey, Bat, Bovine, Dog, Goat, Horse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 - 10 µg/ml)
  • Western blot (4 µg/ml)
  • Gel shift
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
An 18 residue synthetic peptide SPEDKRAGGEESQFEMDI based on the human PHAS-I (residues 101-118) and the peptide coupled to KLH. This sequence is identical to rat and mouse PHAS- I over these residues. Percent identity by BLAST analysis: Human, Gorilla, Mouse, Rat, Hamster, Rabbit (100%); Marmoset, Goat, Elephant, Dog, Bat, Bovine, Horse (94%); Panda (83%).
Epitope
aa101-118
Specificity
Detects multiple bands at 19-25 kD, corresponding to the apparent molecular mass of the various phosphorylation states of PHAS-I protein on SDS-PAGE immunoblots. This antibody provides a simple read-out for PKB (Akt) activity in vivo through the use of gel-shift assays. Antibody specificity has been confirmed in Western blot peptide competition studies.
Usage
Immunohistochemistry: LS-B3748 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3748 was determined to be 10 ug/ml.
Presentation
BBS, pH 8.4 (25 mM sodium borate, 100 mM boric acid, 75 mM sodium chloride, 5 mM EDTA)
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Immunohistochemistry

Anti-EIF4EBP1 / 4EBP1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3748 concentration 5 ug/ml.
Anti-EIF4EBP1 / 4EBP1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3748 concentration 5 ug/ml.

Immunohistochemistry

Anti-EIF4EBP1 / 4EBP1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3748 concentration 5 ug/ml.
Anti-EIF4EBP1 / 4EBP1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3748 concentration 5 ug/ml.

Immunohistochemistry

Anti-EIF4EBP1 / 4EBP1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3748 concentration 5 ug/ml.
Anti-EIF4EBP1 / 4EBP1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3748 concentration 5 ug/ml.

Immunohistochemistry

Anti-EIF4EBP1 / 4EBP1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3748 concentration 5 ug/ml.
Anti-EIF4EBP1 / 4EBP1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3748 concentration 5 ug/ml.

Immunohistochemistry

Anti-EIF4EBP1 / 4EBP1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3748 concentration 5 ug/ml.
Anti-EIF4EBP1 / 4EBP1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3748 concentration 5 ug/ml.

Immunohistochemistry

Anti-EIF4EBP1 / 4EBP1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3748 concentration 5 ug/ml.
Anti-EIF4EBP1 / 4EBP1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3748 concentration 5 ug/ml.

Immunohistochemistry

Anti-EIF4EBP1 / 4EBP1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3748 concentration 5 ug/ml.
Anti-EIF4EBP1 / 4EBP1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3748 concentration 5 ug/ml.

Immunohistochemistry

Anti-EIF4EBP1 / 4EBP1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3748 concentration 5 ug/ml.
Anti-EIF4EBP1 / 4EBP1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3748 concentration 5 ug/ml.


Requested From: 
Date Requested: 6/19/2018

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