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Anti-EIF3S5 / EIF3F Antibody (aa114-125) IHC-plus™ LS-B366

Note: This antibody replaces LS-C18908

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular EIF3S5 / EIF3F Antibodies

Anti-EIF3S5 / EIF3F Antibody (aa114-125) IHC-plus™ LS-B366
Rabbit Polyclonal to Human EIF3S5 / EIF3F
Human, Mouse, Rat, Bovine, Chicken
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-EIF3S5 / EIF3F Antibody (aa93-123) LS-C167694
Rabbit Polyclonal to Human EIF3S5 / EIF3F
Human
IHC, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human EIF3S5 / EIF3F
Human, Mouse, Rat, Bovine, Chicken
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human EIF3S5 / EIF3F
Rabbit
Human, Mouse, Rat, Bovine, Chicken (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • Immunofluorescence
  • Western blot (1:200 - 1:2000)
  • Immunoprecipitation
  • ELISA (1:10000 - 1:40000)

Specificity and Use

EIF3S5 / EIF3F antibody was raised against synthetic peptide from human EIF3F.
aa114-125
Amino acids 114-125 of human eIF3f protein.
Immunohistochemistry: LS-B366 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B366 was determined to be 2.5 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About EIF3S5 / EIF3F

O00303 NM_003754 NP_003745.1

EIF3F Antibody, Deubiquitinating enzyme eIF3f Antibody, EIF-3-epsilon Antibody, EIF3 p47 Antibody, EIF3S5 Antibody, EIF3-epsilon Antibody, EIF3-p47 Antibody

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition.

Immunohistochemistry

Immunohistochemistry
Anti-EIF3F antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B366 concentration 5 ug/ml.

Western blot

Western blot
Anti-eIF3F Antibody - Western Blot. Western blot of affinity purified anti-eIF3f antibody shows detection of endogenous eIF3f in lysates from both control HeLa cells (lane 1) and HeLa cells transformed with the kinase cdk11 (lane 2). Cdk11 is responsible for phosphorylating eIF3f in vivo. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:200. This antibody recognizes both phosphorylated and non-phosphorylated eIF3f. Personal Communication, Jiaqi Shi, Univ. Arizona, Tucson, AZ.

Requested From: United States
Date Requested: 12/7/2016

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