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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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EIF3S5 / EIF3F

eukaryotic translation initiation factor 3, subunit F

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Gene Name: eukaryotic translation initiation factor 3, subunit F
Synonyms: EIF3F, Deubiquitinating enzyme eIF3f, EIF-3-epsilon, EIF3 p47, EIF3S5, EIF3-epsilon, EIF3-p47
Target Sequences: NM_003754 NP_003745.1 O00303

Filtered By: None
Target
EIF3S5 / EIF3F (26)
Reactivity
Human (26)
Mouse (18)
Rat (14)
Bat (8)
Bovine (9)
Chicken (1)
Chimpanzee (6)
Dog (4)
Gibbon (6)
Guinea pig (4)
Horse (4)
Monkey (9)
Rabbit (8)
Application
IHC (12)
IHC-P (8)
WB (25)
Flo (1)
ELISA (6)
IF (2)
IP (4)
Peptide-ELISA (1)
Host
rabbit (25)
mouse (1)
Isotype
IgG (9)
Clonality
polyclonal pc (26)
Format
Biotin Conjugated (3)
FITC Conjugated (3)
HRP Conjugated (3)
Unconjugated (17)
Epitope
Internal (5)
aa279-328 (4)
aa97-146 (4)
50-130 aa, Internal (1)
Val116 (1)
aa114-125 (1)
aa307-357 (1)
aa81-130 (1)
aa93-123 (1)
Publications
No (26)
Showing 1-25 of 26 results

EIF3S5 / EIF3F Antibody (aa114-125) IHC-plus™ LS-B366
Rabbit Polyclonal to Human EIF3S5 / EIF3F
Chicken, Bovine, Mouse, Rat, Human
ELISA IF IHC IHC-P IP WB
Unconjugated
50µg 1mg/ml  $425.00
Anti-EIF3F antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-eIF3F Antibody - Western Blot. Western blot of affinity purified anti-eIF3f antibody shows detection of endogenous eIF3f in lysates from both control HeLa cells (lane 1) and HeLa cells transformed with the kinase cdk11 (lane 2). Cdk11 is responsible for phosphorylating eIF3f in vivo. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:200. This antibody recognizes both phosphorylated and non-phosphorylated eIF3f. Personal Communication, Jiaqi Shi, Univ. Arizona, Tucson, AZ.
Anti-EIF3F antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-eIF3F Antibody - Western Blot. Western blot of affinity purified anti-eIF3f antibody shows detection of endogenous eIF3f in lysates from both control HeLa cells (lane 1) and HeLa cells transformed with the kinase cdk11 (lane 2). Cdk11 is responsible for phosphorylating eIF3f in vivo. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:200. This antibody recognizes both phosphorylated and non-phosphorylated eIF3f. Personal Communication, Jiaqi Shi, Univ. Arizona, Tucson, AZ.

EIF3S5 / EIF3F Antibody IHC-plus™ LS-B15312
Rabbit Polyclonal (IgG) to Human EIF3S5 / EIF3F
Mouse, Human
ELISA IHC IHC-P WB
Unconjugated
60µl  $425.00
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE), at a concentration of 5 ug/ml
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE), at a concentration of 5 ug/ml
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE), at a concentration of 5 ug/ml
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE), at a concentration of 5 ug/ml

EIF3S5 / EIF3F Antibody (aa307-357) IHC-plus™ LS-B15077
Rabbit Polyclonal (IgG) to Human EIF3S5 / EIF3F
Mouse, Human
IHC IHC-P IP WB
Unconjugated
100µl 0.2mg/ml  $485.00
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Detection of Human and Mouse eIF3F by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-eIF3F antibody used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. eIF3F was also immunoprecipitated by rabbit anti-eIF3F antibody BL10962, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Detection of Human and Mouse eIF3F by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-eIF3F antibody used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. eIF3F was also immunoprecipitated by rabbit anti-eIF3F antibody BL10962, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

EIF3S5 / EIF3F Antibody IHC-plus™ LS-B15452
Rabbit Polyclonal (IgG) to Human EIF3S5 / EIF3F
Mouse, Rat, Human
IHC IHC-P WB
Unconjugated
50µl  $425.00
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of extracts of various cell lines.
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of extracts of various cell lines.

EIF3S5 / EIF3F Antibody LS-C675702
Rabbit Polyclonal (IgG) to Human EIF3S5 / EIF3F
Human
ELISA IHC IHC-P IP
Unconjugated
50µl  $235.00; 100µl  $285.00
Immunohistochemistry of paraffin-embedded human adrenal gland tissue at dilution 1:100
Immunohistochemistry of paraffin-embedded human brain tissue at dilution 1:100
Immunohistochemistry of paraffin-embedded human adrenal gland tissue at dilution 1:100
Immunohistochemistry of paraffin-embedded human brain tissue at dilution 1:100

EIF3S5 / EIF3F Antibody (aa93-123) LS-C167694
Rabbit Polyclonal to Human EIF3S5 / EIF3F
Human
Flo IHC WB
Unconjugated
50µg  $250.00
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with EIF3S5 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot of EIF3S5 Antibody (N-term) in A2058 cell line lysates (35 ug/lane). EIF3S5 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with EIF3S5 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot of EIF3S5 Antibody (N-term) in A2058 cell line lysates (35 ug/lane). EIF3S5 (arrow) was detected using the purified Pab.

EIF3S5 / EIF3F Antibody LS-C675701
Rabbit Polyclonal (IgG) to Human EIF3S5 / EIF3F
Human
ELISA IHC IHC-P WB
Unconjugated
50µl  $235.00; 100µl  $285.00
Immunohistochemistry of paraffin-embedded human adrenal gland tissue at dilution 1:100
Western blot All Lanes: EIF3Fantibody at 4.31ug/ml+ Mouse heart tissue Goat polyclonal to rabbit at 1/10000 dilution Predicted band size: 38 kDa Observed band size: 38 kDa
Immunohistochemistry of paraffin-embedded human adrenal gland tissue at dilution 1:100
Western blot All Lanes: EIF3Fantibody at 4.31ug/ml+ Mouse heart tissue Goat polyclonal to rabbit at 1/10000 dilution Predicted band size: 38 kDa Observed band size: 38 kDa

EIF3S5 / EIF3F Antibody LS-C404926
Rabbit Polyclonal (IgG) to Human EIF3S5 / EIF3F
Mouse, Human
ELISA IHC WB
Unconjugated
20µl  $205.00; 60µl  $235.00; 120µl  $295.00; 200µl  $395.00
Immunohistochemistry of Human colon cancer using EIF3F Polyclonal Antibody at dilution of 1:30.
Immunohistochemistry of Human thyroid cancer using EIF3F Polyclonal Antibody at dilution of 1:30.
Immunohistochemistry of Human colon cancer using EIF3F Polyclonal Antibody at dilution of 1:30.
Immunohistochemistry of Human thyroid cancer using EIF3F Polyclonal Antibody at dilution of 1:30.

EIF3S5 / EIF3F Antibody (aa81-130) LS-C119967
Rabbit Polyclonal (IgG) to Human EIF3S5 / EIF3F
Mouse, Rat, Human
IHC IHC-P Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using EIF3F Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HeLa cells, using EIF3F Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using EIF3F Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HeLa cells, using EIF3F Antibody. The lane on the right is blocked with the synthesized peptide.

EIF3S5 / EIF3F Antibody (50-130 aa, Internal) LS-C383300
Rabbit Polyclonal (IgG) to Human EIF3S5 / EIF3F
Mouse, Rat, Human
ELISA IHC WB
Unconjugated
100µl 1mg/ml  $265.00
Western blot of eIF3epsilon antibody
Western blot of eIF3epsilon antibody

EIF3S5 / EIF3F Antibody (Val116) LS-C177451
Rabbit Polyclonal to Human EIF3S5 / EIF3F
Mouse, Human
IHC IHC-P WB
Unconjugated
100µl  $345.00
IHC of eIF3 (V116) pAb in paraffin-embedded human brain tissue.
Western blot of eIF3 (V116) pAb in extracts from HepG2 cells.
IHC of eIF3 (V116) pAb in paraffin-embedded human brain tissue.
Western blot of eIF3 (V116) pAb in extracts from HepG2 cells.

EIF3S5 / EIF3F Antibody LS-C412671
Rabbit Polyclonal to Human EIF3S5 / EIF3F
Mouse, Rat, Human
IHC WB
Unconjugated
100µl  $385.00

EIF3S5 / EIF3F Antibody LS-C307371
Mouse Polyclonal (IgG) to Human EIF3S5 / EIF3F
Human
IF WB
Unconjugated
50µg  $560.00

EIF3S5 / EIF3F Antibody (Internal) LS-C353077
Rabbit Polyclonal to Human EIF3S5 / EIF3F
Mouse, Rat, Human, Monkey
IP WB
Unconjugated
100µl 1mg/ml  $245.00
Western blot analysis of EIF3F expression in HeLa (A); HepG2 (B); NIH3T3 (C) whole cell lysates.
Western blot analysis of EIF3F expression in HeLa (A); HepG2 (B); NIH3T3 (C) whole cell lysates.

EIF3S5 / EIF3F Antibody (Internal) LS-C345791
Rabbit Polyclonal to Human EIF3S5 / EIF3F
Human
WB
Unconjugated
50µg  $365.00

EIF3S5 / EIF3F Antibody (Internal, HRP) LS-C480756
Rabbit Polyclonal to Human EIF3S5 / EIF3F
Human
WB
HRP Conjugated
100µl 0.65mg/ml  $385.00

EIF3S5 / EIF3F Antibody (Internal, Biotin) LS-C480754
Rabbit Polyclonal to Human EIF3S5 / EIF3F
Human
WB
Biotin Conjugated
100µl 1mg/ml  $385.00

EIF3S5 / EIF3F Antibody (Internal, FITC) LS-C480755
Rabbit Polyclonal to Human EIF3S5 / EIF3F
Human
WB
FITC Conjugated
100µl 1mg/ml  $385.00

EIF3S5 / EIF3F Antibody (aa97-146) LS-C145578
Rabbit Polyclonal to Human EIF3S5 / EIF3F
Rabbit, Mouse, Guinea pig, Bovine, Rat, Horse, Bat, Human, Monkey
WB
Unconjugated
50µg  $365.00
EIF3F antibody Western blot of HeLa Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EIF3F antibody Western blot of HeLa Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

EIF3S5 / EIF3F Antibody (aa97-146, HRP) LS-C469229
Rabbit Polyclonal to Human EIF3S5 / EIF3F
Rabbit, Mouse, Guinea pig, Bovine, Rat, Horse, Bat, Gibbon, Chimpanzee, Human, Monkey
WB
HRP Conjugated
100µl 0.65mg/ml  $385.00
EIF3F antibody Western blot of HeLa Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EIF3F antibody Western blot of HeLa Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

EIF3S5 / EIF3F Antibody (aa97-146, Biotin) LS-C469227
Rabbit Polyclonal to Human EIF3S5 / EIF3F
Rabbit, Mouse, Guinea pig, Bovine, Rat, Horse, Bat, Gibbon, Chimpanzee, Human, Monkey
WB
Biotin Conjugated
100µl 1mg/ml  $385.00
EIF3F antibody Western blot of HeLa Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EIF3F antibody Western blot of HeLa Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

EIF3S5 / EIF3F Antibody (aa97-146, FITC) LS-C469228
Rabbit Polyclonal to Human EIF3S5 / EIF3F
Rabbit, Mouse, Guinea pig, Bovine, Rat, Horse, Bat, Gibbon, Chimpanzee, Human, Monkey
WB
FITC Conjugated
100µl 1mg/ml  $385.00
EIF3F antibody Western blot of HeLa Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EIF3F antibody Western blot of HeLa Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

EIF3S5 / EIF3F Antibody (aa279-328) LS-C145579
Rabbit Polyclonal to Human EIF3S5 / EIF3F
Rabbit, Mouse, Dog, Bovine, Rat, Bat, Human, Monkey
WB
Unconjugated
50µg  $365.00
EIF3F antibody Western blot of Fetal Heart lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EIF3F antibody Western blot of Fetal Heart lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

EIF3S5 / EIF3F Antibody (aa279-328, HRP) LS-C469232
Rabbit Polyclonal to Human EIF3S5 / EIF3F
Rabbit, Mouse, Dog, Bovine, Rat, Bat, Gibbon, Chimpanzee, Human, Monkey
WB
HRP Conjugated
100µl 0.65mg/ml  $385.00
EIF3F antibody Western blot of Fetal Heart lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EIF3F antibody Western blot of Fetal Heart lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

EIF3S5 / EIF3F Antibody (aa279-328, FITC) LS-C469231
Rabbit Polyclonal to Human EIF3S5 / EIF3F
Rabbit, Mouse, Dog, Bovine, Rat, Bat, Gibbon, Chimpanzee, Human, Monkey
WB
FITC Conjugated
100µl 1mg/ml  $385.00
EIF3F antibody Western blot of Fetal Heart lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EIF3F antibody Western blot of Fetal Heart lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Showing 1-25 of 26 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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