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Anti-EIF3E Antibody (C-Terminus) LS-C154233

Ordering

Wt. Vol. Conc. Price
100 µg - - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular EIF3E Antibodies

Anti-EIF3E Antibody (aa36-85) IHC-plus™ LS-B5810
Rabbit Polyclonal to Human EIF3E
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-EIF3E Antibody IHC-plus™ LS-B8930
Rabbit Polyclonal to Human EIF3E
Human
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-EIF3E Antibody IHC-plus™ LS-B8931
Rabbit Polyclonal to Human EIF3E
Human
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Mouse EIF3E
Mouse, Human, Monkey, Rat, Bovine, Dog
Western blot, ELISA
Unconjugated

Details

Mouse EIF3E
Rabbit
Mouse, Human, Monkey, Rat, Bovine, Dog (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1:1000)
  • ELISA

Specificity and Use

EIF3E antibody was raised against this affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of mouse EIF3S6/Int6.
C-Terminus
This antibody is specific for mouse eIF3S6/Int6 protein. A BLAST analysis was used to suggest cross-reactivity with most eIF3Se/Int6 isoforms from mouse, human, rat, dog, bovine, and monkey based on 100% homology with the immunizing sequence. Cross-reactivity with EIF3S6/Int6 from other sources has not been determined.
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kD in size corresponding to eIF3S6/Int6 by western blotting in the appropriate cell lysate or extract. This antibody is capable of detecting both over-expressed and endogenous eIF3S6/Int6.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About EIF3E

P60228 NM_001568 NP_001559.1

EIF3E Antibody, EIF3-p46 Antibody, INT6 Antibody, EIF-3 p48 Antibody, EIF3-P48 Antibody, EIF3S6 Antibody

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition.

Western blot

Western blot
Anti-eIF3S6/Int6 Antibody - Western Blot. Western blot of affinity purified anti-eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6. Specific staining is not present in lysates containing lentiviral knockdown vectors (shRNA #1 and #2. Control vectors, specifically a scrambled sequence (Ctl NS) and a sequence against an unrelated gene (Ctl #2), were also used. Personal communication, J. Lee, NCI, Bethesda, MD.

Western blot

Western blot
Anti-eIF3S6/Int6 Antibody - Western Blot. Western blot of affinity purified anti-eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6 in whole cell extracts from murine (HC-11 and NIH3T3), monkey (CV-1 and Cos-1), and human (HEK293T) cell lines as well as over-expressed eIF3S6/Int6 (control transfected flag-tagged Int6). The identity of the higher and lower molecular weight bands is unknown. The band at ~48 kD, indicated by the arrowhead, corresponds to flag-tagged EIF3S6/Int6. Primary antibody was used at 1:1000. Personal communication, J. Lee, NCI, Bethesda, MD.

Requested From: United States
Date Requested: 12/4/2016

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