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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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EIF3E

eukaryotic translation initiation factor 3, subunit E

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.

Gene Name: eukaryotic translation initiation factor 3, subunit E
Synonyms: EIF3E, EIF3-p46, INT6, EIF-3 p48, EIF3-P48, EIF3S6
Target Sequences: NM_001568 NP_001559.1 P60228

Filtered By: None
Target
EIF3E (31)
Reactivity
Human (30)
Mouse (16)
Rat (15)
Bat (9)
Bovine (12)
Chicken (4)
Chimpanzee (6)
Cow (1)
Dog (13)
Gibbon (6)
Guinea pig (10)
Hamster (1)
Horse (11)
Monkey (11)
Orangutan (6)
Pig (10)
Rabbit (11)
Xenopus (4)
Zebrafish (9)
Application
IHC (12)
IHC-P (8)
WB (30)
ELISA (12)
ICC (1)
IF (6)
IP (4)
Peptide-ELISA (1)
Host
rabbit (30)
goat (1)
Isotype
IgG (19)
Clonality
polyclonal pc (31)
Format
AP Conjugated (1)
APC Conjugated (1)
Azide-free (1)
Biotin Conjugated (3)
FITC Conjugated (3)
HRP Conjugated (3)
PE Conjugated (1)
Unconjugated (19)
Epitope
aa248-276 (8)
aa36-85 (4)
aa360-409 (4)
C-Terminus (1)
aa1-445 (1)
aa1-50 (1)
aa425-436 (1)
aa50-100 (1)
Publications
No (31)
Showing 1-25 of 31 results

EIF3E Antibody (aa36-85) IHC-plus™ LS-B5810
Rabbit Polyclonal to Human EIF3E
Zebrafish, Pig, Horse, Bat, Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Human, Monkey
IHC IHC-P WB
Unconjugated
50µg  $485.00
Anti-EIF3E antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EIF3E antibody Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-EIF3E antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EIF3E antibody Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

EIF3E Antibody (aa36-85, HRP) LS-C450819
Rabbit Polyclonal to Human EIF3E
Zebrafish, Pig, Horse, Rabbit, Mouse, Dog, Guinea pig, Bovine, Bat, Rat, Gibbon, Human, Monkey, Orangutan, Chimpanzee
IHC IHC-P WB
HRP Conjugated
100µl 0.65mg/ml  $385.00
Anti-EIF3E antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EIF3E antibody Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-EIF3E antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EIF3E antibody Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

EIF3E Antibody (aa36-85, Biotin) LS-C450526
Rabbit Polyclonal to Human EIF3E
Zebrafish, Pig, Horse, Rabbit, Mouse, Dog, Guinea pig, Bovine, Bat, Rat, Gibbon, Human, Monkey, Orangutan, Chimpanzee
IHC IHC-P WB
Biotin Conjugated
100µl 1mg/ml  $385.00
Anti-EIF3E antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EIF3E antibody Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-EIF3E antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EIF3E antibody Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

EIF3E Antibody (aa36-85, FITC) LS-C450818
Rabbit Polyclonal to Human EIF3E
Zebrafish, Pig, Horse, Rabbit, Mouse, Dog, Guinea pig, Bovine, Bat, Rat, Gibbon, Human, Monkey, Orangutan, Chimpanzee
IHC IHC-P WB
FITC Conjugated
100µl 1mg/ml  $385.00
Anti-EIF3E antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EIF3E antibody Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-EIF3E antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EIF3E antibody Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

EIF3E Antibody IHC-plus™ LS-B8930
Rabbit Polyclonal to Human EIF3E
Human
IHC IHC-P IP WB
Unconjugated
100µg  $485.00
Anti-EIF3E antibody IHC staining of human breast, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-EIF3E antibody IHC staining of human breast, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

EIF3E Antibody IHC-plus™ LS-B8931
Rabbit Polyclonal to Human EIF3E
Human
IHC IHC-P IP WB
Unconjugated
100µg  $485.00
Human Pancreas, Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Pancreas, Formalin-Fixed, Paraffin-Embedded (FFPE)

EIF3E Antibody LS-C753193
Rabbit Polyclonal (IgG) to Human EIF3E
Human
ELISA IF IHC WB
Unconjugated
100µg  $355.00

EIF3E Antibody LS-C753194
Rabbit Polyclonal (IgG) to Human EIF3E
Human
ELISA IF IHC WB
Unconjugated
100µg  $335.00

EIF3E Antibody (aa50-100) LS-C289694
Rabbit Polyclonal (IgG) to Human EIF3E
Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Pig, Horse, Human
IF IHC IHC-P IP WB
Unconjugated
100µl 0.2mg/ml  $395.00
Detection of Human eIF3E by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-eIF3E used at a dilution of 1:200 (1 ug/ml). Detection: DAB.
Detection of Human eIF3E by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-eIF3E used at a dilution of 1:200 (1 ug/ml). Detection: DAB.

EIF3E Antibody LS-C346085
Rabbit Polyclonal (IgG) to Human EIF3E
Mouse, Human
IF IHC WB
Unconjugated
50µl  $235.00; 100µl  $315.00; 200µl  $425.00
Immunofluorescence analysis of HeLa cells.
Western blot analysis of extracts of various cells.
Immunofluorescence analysis of HeLa cells.
Western blot analysis of extracts of various cells.

EIF3E Antibody LS-C482444
Rabbit Polyclonal to Human EIF3E
Mouse, Rat, Human
ICC IF WB
Unconjugated
50µl 1mg/ml  $255.00; 100µl 1mg/ml  $295.00; 200µl 1mg/ml  $375.00
Immunofluorescent analysis of eIF3E staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Western blot analysis of eIF3E expression in MCF7 (A); K562 (B); HepG2 (C) whole cell lysates.
Immunofluorescent analysis of eIF3E staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Western blot analysis of eIF3E expression in MCF7 (A); K562 (B); HepG2 (C) whole cell lysates.

EIF3E Antibody (aa1-445) LS-C375645
Rabbit Polyclonal (IgG) to Human EIF3E
Human
ELISA IHC IHC-P
Unconjugated
50µl  $285.00; 100µl  $385.00
Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.
Immunohistochemistry of paraffin-embedded human kidney using antibody at 1:100 dilution.
Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.
Immunohistochemistry of paraffin-embedded human kidney using antibody at 1:100 dilution.

EIF3E Antibody LS-C170819
Rabbit Polyclonal to Human EIF3E
IF WB
Unconjugated
100µg  $365.00

EIF3E Antibody LS-C341797
Rabbit Polyclonal to Human EIF3E
Mouse, Rat, Human
IHC WB
Unconjugated
100µl 1mg/ml  $385.00

EIF3E Antibody (C-Terminus) LS-C154233
Rabbit Polyclonal (IgG) to Mouse EIF3E
Mouse, Dog, Bovine, Rat, Human, Monkey
ELISA WB
Unconjugated
100µg  $425.00
Anti-eIF3S6/Int6 Antibody - Western Blot. Western blot of affinity purified anti-eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6 in whole cell extracts from murine (HC-11 and NIH3T3), monkey (CV-1 and Cos-1), and human (HEK293T) cell lines as well as over-expressed eIF3S6/Int6 (control transfected flag-tagged Int6). The identity of the higher and lower molecular weight bands is unknown. The band at ~48 kD, indicated by the arrowhead, corresponds to flag-tagged EIF3S6/Int6. Primary antibody was used at 1:1000. Personal communication, J. Lee, NCI, Bethesda, MD.
Anti-eIF3S6/Int6 Antibody - Western Blot. Western blot of affinity purified anti-eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6. Specific staining is not present in lysates containing lentiviral knockdown vectors (shRNA #1 and #2. Control vectors, specifically a scrambled sequence (Ctl NS) and a sequence against an unrelated gene (Ctl #2), were also used. Personal communication, J. Lee, NCI, Bethesda, MD.
Anti-eIF3S6/Int6 Antibody - Western Blot. Western blot of affinity purified anti-eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6 in whole cell extracts from murine (HC-11 and NIH3T3), monkey (CV-1 and Cos-1), and human (HEK293T) cell lines as well as over-expressed eIF3S6/Int6 (control transfected flag-tagged Int6). The identity of the higher and lower molecular weight bands is unknown. The band at ~48 kD, indicated by the arrowhead, corresponds to flag-tagged EIF3S6/Int6. Primary antibody was used at 1:1000. Personal communication, J. Lee, NCI, Bethesda, MD.
Anti-eIF3S6/Int6 Antibody - Western Blot. Western blot of affinity purified anti-eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6. Specific staining is not present in lysates containing lentiviral knockdown vectors (shRNA #1 and #2. Control vectors, specifically a scrambled sequence (Ctl NS) and a sequence against an unrelated gene (Ctl #2), were also used. Personal communication, J. Lee, NCI, Bethesda, MD.

EIF3E Antibody LS-C745313
Rabbit Polyclonal (IgG) to Mouse EIF3E
Mouse, Dog, Rat, Cow, Human, Monkey
ELISA WB
Unconjugated
25µl  $235.00
Western blot using the affinity purified anti-eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6 in whole cell extracts from murine (HC-11 and NIH3T3), monkey (CV-1 and Cos-1), and human (HEK293T) cell lines as well as over-expressed eIF3S6/Int6 (control transfected flag-tagged Int6).  The identity of the higher and lower molecular weight bands is unknown.  The band at ~48 kDa, indicated by the arrowhead, corresponds to flag-tagged EIF3S6/Int6.  Primary antibody was used at 1:1000.
Western blot using the affinity purified anti-eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6. Specific staining is not present in lysates containing lentiviral knockdown vectors (shRNA #1 and #2). Control vectors, specifically a scrambled sequence (Ctl NS) and a sequence against an unrelated gene (Ctl #2), were also used.
Western blot using the affinity purified anti-eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6 in whole cell extracts from murine (HC-11 and NIH3T3), monkey (CV-1 and Cos-1), and human (HEK293T) cell lines as well as over-expressed eIF3S6/Int6 (control transfected flag-tagged Int6).  The identity of the higher and lower molecular weight bands is unknown.  The band at ~48 kDa, indicated by the arrowhead, corresponds to flag-tagged EIF3S6/Int6.  Primary antibody was used at 1:1000.
Western blot using the affinity purified anti-eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6. Specific staining is not present in lysates containing lentiviral knockdown vectors (shRNA #1 and #2). Control vectors, specifically a scrambled sequence (Ctl NS) and a sequence against an unrelated gene (Ctl #2), were also used.

EIF3E Antibody (aa248-276) LS-C321065
Rabbit Polyclonal (IgG) to Human EIF3E
Human
ELISA WB
Unconjugated
200µl  $530.00

EIF3E Antibody (aa248-276, Biotin) LS-C213733
Rabbit Polyclonal (IgG) to Human EIF3E
Human
ELISA WB
Biotin Conjugated
200µl  $680.00

EIF3E Antibody (aa248-276, FITC) LS-C213734
Rabbit Polyclonal (IgG) to Human EIF3E
Human
ELISA WB
FITC Conjugated
200µl  $680.00

EIF3E Antibody (aa248-276, APC) LS-C213731
Rabbit Polyclonal (IgG) to Human EIF3E
Human
ELISA WB
APC Conjugated
200µl  $680.00

EIF3E Antibody (aa248-276, AP) LS-C213732
Rabbit Polyclonal (IgG) to Human EIF3E
Human
ELISA WB
AP Conjugated
200µl  $680.00

EIF3E Antibody (aa248-276, PE) LS-C213735
Rabbit Polyclonal (IgG) to Human EIF3E
Human
ELISA WB
PE Conjugated
200µl  $680.00

EIF3E Antibody (aa248-276, Azide-free, HRP) LS-C213736
Rabbit Polyclonal (IgG) to Human EIF3E
Human
ELISA WB
HRP Conjugated, Azide-free
200µl  $680.00

EIF3E Antibody (aa1-50) LS-C289693
Rabbit Polyclonal (IgG) to Human EIF3E
Zebrafish, Horse, Rat, Rabbit, Mouse, Dog, Guinea pig, Bovine, Human
IP WB
Unconjugated
100µl 1mg/ml  $395.00

EIF3E Antibody (aa425-436) LS-C139691
Goat Polyclonal to Human EIF3E
Rabbit, Mouse, Dog, Bovine, Rat, Hamster, Pig, Horse, Bat, Human, Monkey
Peptide-ELISA WB
Unconjugated
100µg  $405.00
EIF3E antibody (0.03 ug/ml) staining of Daudi lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EIF3E antibody (0.03 ug/ml) staining of Daudi lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Showing 1-25 of 31 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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