Human EIF2B5

Rabbit

Human, Rat (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Synthetic phosphopeptide derived from the region of rat eIF2B that contains serine 535 (corresponding to serine 539 in the human sequence).

pSer539

Human (80% homologous) and rat eIF2B.

• Western blot
• Functional Assay

The antibody has been used in Western blotting. Eukaryotic Initiation Factor 2B (eIF2B) plays a key role in the initiation of mRNA translation in eukaryotic cells by acting as a guanine nucleotide exchange factor. The activity of eIF2B is regulated by a wide array of conditions. Stress inhibits its action, while stimulation of protein synthesis results in its activation. eIF2B, an 82kD subunit of eIF2B, is phosphorylated at serine 539 by glycogen synthase kinase-3 (GSK-3), which leads to inhibition of its guanine nucleotide exchange activity. Positive control: Recombinant eIF2B treated with GSK-3 ; CHO-T cells transfected with human insulin receptor (IR) +/- insulin.

1 mg/ml BSA (IgG, Protease free), PBS (without Mg2+, Ca2+), pH 7.2, 0.1% Sodium Azide, 50% Glycerol

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q13144 NCBI: U23028 AAC50646.1