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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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EIF2AK3 / PERK Antibody (aa148‑175) LS‑C101136
PERK antibody LS-C101136 is an unconjugated rabbit polyclonal antibody to human PERK (EIF2AK3). Validated for IHC and WB.
Catalog
Size
Price
LS-C101136-400
400 µl
Unavailable

Popular EIF2AK3 / PERK Products

Antibody
Species: Mouse, Rat
Applications: Western blot, ELISA
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Species: Human, Mouse, Rat
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Western blot using EIF2AK3 monoclonal antibody against HEK293 (1) and EIF2AK3(AA: 929-1116)-hIgGFc transfected HEK293 (2) cell lysate.
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Applications: Western blot, ELISA
Immunohistochemical analysis of PERK staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation

Product Description

PERK antibody LS-C101136 is an unconjugated rabbit polyclonal antibody to human PERK (EIF2AK3). Validated for IHC and WB.
About EIF2AK3 / PERK
Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function. Q9NZJ5 NM_004836 NP_004827.4

EIF2AK3 Antibody, HRI Antibody, HsPEK Antibody, Pancreatic eIF2-alpha kinase Antibody, PEK Antibody, PERK Antibody, PKR-like ER kinase Antibody, WRS Antibody


Specifications

Target
Human EIF2AK3 / PERK
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E9458 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C101136. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa148-175
Specificity
This PERK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 148-175 amino acids from the N-terminal region of human PERK.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PERK antibody (N-term Q163) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PERK antibody (N-term Q163) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of PERK Antibody (N-term Q163) in 293 cell line lysates (35 ug/lane). PERK (arrow) was detected using the purified antibody.
Western blot of PERK Antibody (N-term Q163) in 293 cell line lysates (35 ug/lane). PERK (arrow) was detected using the purified antibody.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PERK antibody (N-term Q163) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PERK antibody (N-term Q163) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of PERK Antibody (N-term Q163) in 293 cell line lysates (35 ug/lane). PERK (arrow) was detected using the purified antibody.
Western blot of PERK Antibody (N-term Q163) in 293 cell line lysates (35 ug/lane). PERK (arrow) was detected using the purified antibody.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PERK antibody (N-term Q163) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PERK antibody (N-term Q163) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of PERK Antibody (N-term Q163) in 293 cell line lysates (35 ug/lane). PERK (arrow) was detected using the purified antibody.
Western blot of PERK Antibody (N-term Q163) in 293 cell line lysates (35 ug/lane). PERK (arrow) was detected using the purified antibody.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PERK antibody (N-term Q163) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PERK antibody (N-term Q163) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of PERK Antibody (N-term Q163) in 293 cell line lysates (35 ug/lane). PERK (arrow) was detected using the purified antibody.
Western blot of PERK Antibody (N-term Q163) in 293 cell line lysates (35 ug/lane). PERK (arrow) was detected using the purified antibody.


Requested From: 
Date Requested: 6/21/2018

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