EGFR antibody LS-B2914 is an unconjugated sheep polyclonal antibody to EGFR from human, mouse, rat and other species. Validated for ICC, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Human, Mouse, Rat, Chicken
(tested or 100% immunogen sequence identity)
Protein G purified
- IHC - Paraffin (5 µg/ml)
- Western blot
- Immunoprecipitation (10 - 12 µg/ml)
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EGFR antibody was raised against a synthetic 20 amino acid peptide of human EGFr representing a portion of the internal domain proximal to a phosphorylation region.
This EGFr antibody recognizes a region of the internal domain. Antibody binding blocks phosphorylation and does not interfere with EGF binding.
Immunohistochemistry: LS-B2914 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2914 was determined to be 5 ug/ml. Western Blot: Dilution range 10 - 50 ug/mL dependent on receptor concentration. Immunoprecipitation (IP) - A431 epidermoid carcinoma 10 - 12 ug/mL. Protein kinase activity is measurable on the immunoprecipitated receptor. Immunocytochemistry (IC) Formalin fixed cells: 25 - 100 ug/mL dependent on receptor concentration.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.