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Anti-EGFR Antibody (Internal) IHC-plus™ LS-B2914

Note: This antibody replaces LS-C84004

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular EGFR Antibodies

Anti-EGFR Antibody (Internal) IHC-plus™ LS-B2199
Sheep Polyclonal (IgG) to Human EGFR
Human, Mouse, Rat, Chicken
IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-EGFR Antibody (clone H11) LS-C26773
Mouse Monoclonal [clone H11] (IgG1)
Human
IHC - Paraffin, Immunofluorescence, Immunoprecipitation, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-EGFR Antibody (Internal) IHC-plus™ LS-B2914
Sheep Polyclonal to Human EGFR
Human, Mouse, Rat, Chicken
IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Sheep Polyclonal to Human EGFR
Human, Mouse, Rat, Chicken
IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Unconjugated

Details

Human EGFR
Sheep
Human, Mouse, Rat, Chicken (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • ICC
  • Western blot
  • Immunoprecipitation (10 - 12 µg/ml)

Specificity and Use

EGFR antibody was raised against a synthetic 20 amino acid peptide of human EGFr representing a portion of the internal domain proximal to a phosphorylation region.
Internal
This EGFr antibody recognizes a region of the internal domain. Antibody binding blocks phosphorylation and does not interfere with EGF binding.
Immunohistochemistry: LS-B2914 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2914 was determined to be 5 ug/ml. Western Blot: Dilution range 10 - 50 ug/mL dependent on receptor concentration. Immunoprecipitation (IP) - A431 epidermoid carcinoma 10 - 12 ug/mL. Protein kinase activity is measurable on the immunoprecipitated receptor. Immunocytochemistry (IC) Formalin fixed cells: 25 - 100 ug/mL dependent on receptor concentration.

Packaging

PBS, pH 7.4.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About EGFR

P00533 NM_005228 NP_005219.2

EGFR Antibody, EGFf receptor Antibody, ERBB Antibody, ERBB1 Antibody, MENA Antibody, PIG61 Antibody, Proto-oncogene c-ErbB-1 Antibody, V-erb-b homolog Antibody, EGF-R Antibody, HER1 Antibody

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues.

Immunohistochemistry

Immunohistochemistry
Anti-EGFR antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2914 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-EGFR antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2914 concentration 5 ug/ml.

Requested From: United States
Date Requested: 12/8/2016

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